LOCUS       sequence-133805-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0 Entry vector"" ORF - eGFP                         "".
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-ORF-eGFP
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..724
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(41..69)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     664..684
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggtctcacat atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
       61 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      121 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      181 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
      241 tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      301 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      361 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      421 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
      481 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
      541 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
      601 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagatca
      661 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
      721 caaataatcg aagagacc
//
