LOCUS       sequence-133808-        2142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 34 to 68)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-J23100
FEATURES             Location/Qualifiers
     source          1..2142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        34..68
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     terminator      102..159
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(183..200)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(354..942)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(434..453)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1125..1219)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1240..1899)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1814..1833
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2061..2079
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2083..2126)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    join(2129..2142,1..6)
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 ttctagaatc tagagacgca taggtctcac tatttgacgg ctagctcagt cctaggtaca
       61 gtgctagcgt acagagaccc atgccgtctc attagctgca gtccggcaaa aaagggcaag
      121 gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct
      181 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      241 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      301 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccac
      361 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      421 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      481 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      541 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      601 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      661 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      721 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      781 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      841 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      901 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      961 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1021 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1081 taaagtatat atgagtaaac ttggtctgac agctcgaggc ttggattctc accaataaaa
     1141 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     1201 ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc
     1261 atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc
     1321 atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc
     1381 catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt
     1441 gaaactcacc cagggattgg ctgacacgaa aaacatattc tcaataaacc ctttagggaa
     1501 ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg
     1561 gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac
     1621 ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg
     1681 aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
     1741 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
     1801 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
     1861 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
     1921 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
     1981 ggaacctctt acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta
     2041 tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat
     2101 ccttagcttt cgctaaggat gatttctgga attcgcggcc gc
//
