LOCUS       sequence-13381-e         405 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins
            LM, Xiao H, Tjandra N, Wu C, Bai Y
  TITLE     Structural basis for recognition of centromere histone variant CenH3
            by the chaperone Scm3.
  JOURNAL   Nature. 2011 Apr 14. 472(7342):234-7.
  PUBMED    21412236
REFERENCE   2  (bases 1 to 405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Apr 14. 472(7342):234-7."
COMMENT     Sequence Label: pET-sC4T
FEATURES             Location/Qualifiers
     source          1..405
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             202..219
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 catatgcacc accaccacca ccacaagaaa ttattaatct ccaaaattcc atttgcaagg
       61 ctagtgaaag aagttacaga cgagtttaca actaaagatc aggatttacg ttggcagtca
      121 atggcgatta tggcgttaca ggaagcaagc gaagcgtatc tggtaggatt attggaacat
      181 acaaacctct tggcgctgca tctggttccg cgtggatcca agcgtatttc tggtttgatc
      241 tacgaagaag tcagagccgt cttgaaatcc ttcttggaat ccgtcatcag ggactctgtt
      301 acttacactg aacacgccaa gagaaagact gttacttctt tggatgttgt ttatgctttg
      361 aagagacaag gtagaacctt atatggtttc ggtggttaac tcgag
//
