LOCUS       sequence-13383-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins
            LM, Xiao H, Tjandra N, Wu C, Bai Y
  TITLE     Structural basis for recognition of centromere histone variant CenH3
            by the chaperone Scm3.
  JOURNAL   Nature. 2011 Apr 14. 472(7342):234-7.
  PUBMED    21412236
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2011 Apr 14. 472(7342):234-7."
COMMENT     Sequence Label: pET-sCS4T+N
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             265..282
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 catatgcacc accaccacca ccacaagaaa ttattaatct ccaaaattcc atttgcaagg
       61 ctagtgaaag aagttacaga cgagtttaca actaaagatc aggatttacg ttggcagtca
      121 atggcgatta tggcgttaca ggaagcaagc gaagcgtatc tggtaggatt attggaacat
      181 acaaacctct tggcgctgca tgcaaaaaga attactataa tgaagaaaga catgcaacta
      241 gcaagaagaa tcaggggaca gtttctggtt ccgcgtggat ccggtggtgg tcctaaacta
      301 accgatgatg aagtcatgga gcgccacaaa ctagcagacg aaaacatgag aaaagtgtgg
      361 tccaacatca taagcaaata cgagtctatt gaagaacaag gagacctcgt agacctgaaa
      421 actggtgaaa tagttgaaga taatgggcat atcaagacgc tgacggccaa caacagcacg
      481 aaagataaaa gaacaaaata cacaagcgtg ctcagagaca ttatcgacat cagcgacgag
      541 gaagatggtg ataaaggtgg tgtcaagcgt atttctggtt tgatctacga agaagtcaga
      601 gccgtcttga aatccttctt ggaatccgtc atcagggact ctgttactta cactgaacac
      661 gccaagagaa agactgttac ttctttggat gttgtttatg ctttgaagag acaaggtaga
      721 accttatatg gtttcggtgg ttaactcgag
//
