LOCUS       sequence-133831-        2124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hexahistidine N-terminal tag (5' TAAA/3' CATA fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-HexHis
FEATURES             Location/Qualifiers
     source          1..2124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             29..46
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      77..134
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(158..175)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(329..917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(409..428)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1100..1194)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1215..1874)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1789..1808
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2036..2054
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2058..2101)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2104..2123
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 atctagagac gcataggtct cataaatgca ccatcaccat caccataaga gacccatgcc
       61 gtctcattag ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt
      121 taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct
      181 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      241 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      301 accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc
      361 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      421 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      481 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      541 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      601 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      661 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      721 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      781 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      841 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      901 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
      961 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1021 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1081 ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     1141 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     1201 agctcgatat caaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta
     1261 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     1321 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     1381 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgac
     1441 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     1501 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     1561 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     1621 catatcacca gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg
     1681 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     1741 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     1801 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     1861 atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     1921 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg cccgatcaac
     1981 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     2041 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt
     2101 ctggaattcg cggccgcttc taga
//
