LOCUS       sequence-133866-        8440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of human VEGFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8440)
  TITLE     Grusch plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQCXIP-VEGFC
FEATURES             Location/Qualifiers
     source          1..8440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1604..1907
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1908..2111
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2108..2132
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    join(3567..4141,4142..4144,4145..4153)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3734..3751)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3961..3980
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4173..4772
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4173..4192)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4669..4689
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5146..5571
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(5674..5696)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5832..5852)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5837..6166
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6017..6152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6188..6207
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6288..6305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6459..7047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6539..6558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7218..8009,8010..8078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7841..7860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8079..8183)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8251..8269
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(8369..8440,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc tgggggatcg atcctctaga gtccgttaca taacttacgg
     1621 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1681 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1741 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1801 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1861 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1921 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1981 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     2041 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     2101 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2161 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
     2221 cggacctgca ggaattgatc cgcggccgca ccggtaggcc tcgtacgctt aattaacgga
     2281 tccatgcact tgctgggctt cttctctgtg gcgtgttctc tgctcgccgc tgcgctgctc
     2341 ccgggtcctc gcgaggcgcc cgccgccgcc gccgccttcg agtccggact cgacctctcg
     2401 gacgcggagc ccgacgcggg cgaggccacg gcttatgcaa gcaaagatct ggaggagcag
     2461 ttacggtctg tgtccagtgt agatgaactc atgactgtac tctacccaga atattggaaa
     2521 atgtacaagt gtcagctaag gaaaggaggc tggcaacata acagagaaca ggccaacctc
     2581 aactcaagga cagaagagac tataaaattt gctgcagcac attataatac agagatcttg
     2641 aaaagtattg ataatgagtg gagaaagact caatgcatgc cacgggaggt gtgtatagat
     2701 gtggggaagg agtttggagt cgcgacaaac accttcttta aacctccatg tgtgtccgtc
     2761 tacagatgtg ggggttgctg caatagtgag gggctgcagt gcatgaacac cagcacgagc
     2821 tacctcagca agacgttatt tgaaattaca gtgcctctct ctcaaggccc caaaccagta
     2881 acaatcagtt ttgccaatca cacttcctgc cgatgcatgt ctaaactgga tgtttacaga
     2941 caagttcatt ccattattag acgttccctg ccagcaacac taccacagtg tcaggcagcg
     3001 aacaagacct gccccaccaa ttacatgtgg aataatcaca tctgcagatg cctggctcag
     3061 gaagatttta tgttttcctc ggatgctgga gatgactcaa cagatggatt ccatgacatc
     3121 tgtggaccaa acaaggagct ggatgaagag acctgtcagt gtgtctgcag agcggggctt
     3181 cggcctgcca gctgtggacc ccacaaagaa ctagacagaa actcatgcca gtgtgtctgt
     3241 aaaaacaaac tcttccccag ccaatgtggg gccaaccgag aatttgatga aaacacatgc
     3301 cagtgtgtat gtaaaagaac ctgccccaga aatcaacccc taaatcctgg aaaatgtgcc
     3361 tgtgaatgta cagaaagtcc acagaaatgc ttgttaaaag gaaagaagtt ccaccaccaa
     3421 acatgcagct gttacagacg gccatgtacg aaccgccaga aggcttgtga gccaggattt
     3481 tcatatagtg aagaagtgtg tcgttgtgtc ccttcatatt ggaaaagacc acaaatgagc
     3541 taagaattcc gccccccccc cccccccccc tctccctccc ccccccctaa cgttactggc
     3601 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     3661 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     3721 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     3781 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     3841 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     3901 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     3961 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     4021 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     4081 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     4141 gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc acggtgcgcc
     4201 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
     4261 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
     4321 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     4381 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     4441 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     4501 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     4561 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     4621 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     4681 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     4741 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     4801 ccgaaaggag cgcacgaccc catggctccg accgaagcca cccggggcgg ccccgccgac
     4861 cccgcacccg cccccgaggc ccaccgactc tagtcgaggg ctgcagcgct gcagaggccg
     4921 agtgcagaac tgctccaaag ggacctcaag gctttccgag ggacactagg ctgactccat
     4981 cgagccagtg tagagataag cttatcgatt agtccaattt gttaaagaca ggatatcagt
     5041 ggtccaggct ctagttttga ctcaacaata tcaccagctg aagcctatag agtacgagcc
     5101 atagataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
     5161 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
     5221 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagggt cgaccctaga
     5281 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     5341 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     5401 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt
     5461 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt
     5521 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc
     5581 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg
     5641 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     5701 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     5761 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
     5821 gatccggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     5881 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     5941 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6001 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6061 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6121 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttactggctt
     6181 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     6241 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
     6301 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6361 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6421 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6481 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6541 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6601 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6661 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6721 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6781 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6841 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     6901 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6961 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7021 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7081 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7141 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7261 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7321 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7381 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7441 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7501 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
     7561 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7621 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7681 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7741 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7801 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
     7861 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7921 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7981 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8041 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     8221 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
     8281 caagaattag cttggccatt gcatacgttg tatccatatc ataatatgta catttatatt
     8341 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa
     8401 tcaattacgg ggtcattagt tcatagccca tatatggagt
//
