LOCUS       sequence-133880-       10710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of HIF2A-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Liu XD, Yao J, Tripathi DN, Ding Z, Xu Y, Sun M, Zhang J, Bai S, 
            German P, Hoang A, Zhou L, Jonasch D, Zhang X, Conti CJ, Efstathiou 
            E, Tannir NM, Eissa NT, Mills GB, Walker CL, Jonasch E
  TITLE     Autophagy mediates HIF2alpha degradation and suppresses renal 
            tumorigenesis.
  JOURNAL   Oncogene. 2015 May 7;34(19):2450-60. doi: 10.1038/onc.2014.199. Epub
            2014 Jul 7.
  PUBMED    24998849
REFERENCE   2  (bases 1 to 10710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2014.199"; journalName: "Oncogene"; date: "2015-05-7- 
            7"; volume: "34"; issue: "19"; pages: "2450-60"
COMMENT     Sequence Label: pCDH-CB1-HIF2a-GFP-T2A-Puro
FEATURES             Location/Qualifiers
     source          1..10710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     intron          2567..2663
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      2731..2740
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2746..2772
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2746..2772
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2752..2778
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             join(5407..5409,5410..5412,5413..6123)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5407..5409,5410..5412,5413..6123)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5452..5473)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5713..5732)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6060..6081
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6139..6192
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6193..6792
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6193..6212)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6689..6709
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6805..7393
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6858..6878)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7276..7287)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7467..7700
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7772..7893
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7809..7828)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7863..7882
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7912..8047
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8085..8101)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8085..8101)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8098..8120)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8109..8125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8133..8139,8140..8157,8158..8163))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8178..8199
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8316..8333)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8487..9075)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9246..10037,10038..10106))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9869..9888
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10107..10211)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10279..10297
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10335..10357)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10457..10476
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10670..10692
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10684..10701
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10685..10701
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 ttaattaagg tacctctggt cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1981 cccaacgacc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2041 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2101 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2161 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctact
     2221 cgaggccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
     2281 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggggggc
     2341 gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg
     2401 cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc
     2461 ggcggcccta taaaaagcga agcgcgcggc gggcgggagc gggatcagcc accgcggtgg
     2521 cggccctaga gtcgatcgag gaactgaaaa accagaaagt taactggtaa gtttagtctt
     2581 tttgtctttt atttcaggtc ccggatccgg tggtggtgca aatcaaagaa ctgctcctca
     2641 gtggatgttg cctttacttc taggcctgta cggaagtgtt acttctgctc taaaagctgc
     2701 ggaattgtac ccgcggccga tccaccggtc gccaccatgg ctagctaccc ctacgacgtg
     2761 cccgactacg cctccctcgg atccgaggcc atgacagctg acaaggagaa gaaaaggagt
     2821 agctcggaga ggaggaagga gaagtcccgg gatgctgcgc ggtgccggcg gagcaaggag
     2881 acggaggtgt tctatgagct ggcccatgag ctgcctctgc cccacagtgt gagctcccat
     2941 ctggacaagg cctccatcat gcgactggca atcagcttcc tgcgaacaca caagctcctc
     3001 tcctcagttt gctctgaaaa cgagtccgaa gccgaagctg accagcagat ggacaacttg
     3061 tacctgaaag ccttggaggg tttcattgcc gtggtgaccc aagatggcga catgatcttt
     3121 ctgtcagaaa acatcagcaa gttcatggga cttacacagg tggagctaac aggacatagt
     3181 atctttgact tcactcatcc ctgcgaccat gaggagattc gtgagaacct gagtctcaaa
     3241 aatggctctg gttttgggaa aaaaagcaaa gacatgtcca cagagcggga cttcttcatg
     3301 aggatgaagt gcacggtcac caacagaggc cgtactgtca acctcaagtc agccacctgg
     3361 aaggtcttgc actgcacggg ccaggtgaaa gtctacaaca actgccctcc tcacaatagt
     3421 ctgtgtggct acaaggagcc cctgctgtcc tgcctcatca tcatgtgtga accaatccag
     3481 cacccatccc acatggacat ccccctggat agcaagacct tcctgagccg ccacagcatg
     3541 gacatgaagt tcacctactg tgatgacaga atcacagaac tgattggtta ccaccctgag
     3601 gagctgcttg gccgctcagc ctatgaattc taccatgcgc tagactccga gaacatgacc
     3661 aagagtcacc agaacttgtg caccaagggt caggtagtaa gtggccagta ccggatgctc
     3721 gcaaagcatg ggggctacgt gtggctggag acccagggga cggtcatcta caaccctcgc
     3781 aacctgcagc cccagtgcat catgtgtgtc aactacgtcc tgagtgagat tgagaagaat
     3841 gacgtggtgt tctccatgga ccagactgaa tccctgttca agccccacct gatggccatg
     3901 aacagcatct ttgatagcag tggcaagggg gctgtgtctg agaagagtaa cttcctattc
     3961 accaagctaa aggaggagcc cgaggagctg gcccagctgg ctcccacccc aggagacgcc
     4021 atcatctctc tggatttcgg gaatcagaac ttcgaggagt cctcagccta tggcaaggcc
     4081 atcctgcccc cgagccagcc atgggccacg gagttgagga gccacagcac ccagagcgag
     4141 gctgggagcc tgcctgcctt caccgtgccc caggcagctg ccccgggcag caccaccccc
     4201 agtgccacca gcagcagcag cagctgctcc acgcccaata gccctgaaga ctattacaca
     4261 tctttggata acgacctgaa gattgaagtg attgagaagc tcttcgccat ggacacagag
     4321 gccaaggacc aatgcagtac ccagacggat ttcaatgagc tggacttgga gacactggca
     4381 ccctatatcc ccatggacgg ggaagacttc cagctaagcc ccatctgccc cgaggagcgg
     4441 ctcttggcgg agaacccaca gtccaccccc cagcactgct tcagtgccat gacaaacatc
     4501 ttccagccac tggcccctgt agccccgcac agtcccttcc tcctggacaa gtttcagcag
     4561 cagctggaga gcaagaagac agagcccgag caccggccca tgtcctccat cttctttgat
     4621 gccggaagca aagcatccct gccaccgtgc tgtggccagg ccagcacccc tctctcttcc
     4681 atggggggca gatccaatac ccagtggccc ccagatccac cattacattt tgggcccaca
     4741 aagtgggccg tcggggatca gcgcacagag ttcttgggag cagcgccgtt ggggccccct
     4801 gtctctccac cccatgtctc caccttcaag acaaggtctg caaagggttt tggggctcga
     4861 ggcccagacg tgctgagtcc ggccatggta gccctctcca acaagctgaa gctgaagcga
     4921 cagctggagt atgaagagca agccttccag gacctgagcg ggggggaccc acctggtggc
     4981 agcacctcac atttgatgtg gaaacggatg aagaacctca ggggtgggag ctgccctttg
     5041 atgccggaca agccactgag cgcaaatgta cccaatgata agttcaccca aaaccccatg
     5101 aggggcctgg gccatcccct gagacatctg ccgctgccac agcctccatc tgccatcagt
     5161 cccggggaga acagcaagag caggttcccc ccacagtgct acgccaccca gtaccaggac
     5221 tacagcctgt cgtcagccca caaggtgtca ggcatggcaa gccggctgct cgggccctca
     5281 tttgagtcct acctgctgcc cgaactgacc agatatgact gtgaggtgaa cgtgcccgtg
     5341 ctgggaagct ccacgctcct gcaaggaggg gacctcctca gagccctgga ccaggccacc
     5401 aagcttatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     5461 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     5521 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     5581 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     5641 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5701 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     5761 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5821 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     5881 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     5941 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     6001 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     6061 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     6121 aaggtcgacg gcagtggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag
     6181 aatcctggcc caatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     6241 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     6301 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     6361 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     6421 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     6481 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     6541 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     6601 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     6661 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     6721 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     6781 cccggtgcct gagaattcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     6841 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     6901 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     6961 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7021 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7081 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7141 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7201 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7261 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7321 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7381 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     7441 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaaaataaga
     7501 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7561 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7621 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7681 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7741 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     7801 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7861 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     7921 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7981 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     8041 ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata gctgtttcct
     8101 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8161 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8221 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8281 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8341 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8401 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8461 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8521 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8581 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8641 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8701 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8761 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8821 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8881 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8941 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9001 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9061 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9121 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9181 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9241 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9301 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9361 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9421 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9481 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9541 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9601 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9661 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9721 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9781 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9841 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9901 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9961 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10021 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10081 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10141 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    10201 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
    10261 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
    10321 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
    10381 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
    10441 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
    10501 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
    10561 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
    10621 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
    10681 cgttgtaaaa cgacggccag tgccaagctg
//
