LOCUS       sequence-133926-        1018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase, Ser57>Val mutant (AaPurE1-S57V).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Kappock
  TITLE     Serine mutants of Acetobacter aceti PurE1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK677
FEATURES             Location/Qualifiers
     source          1..1018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..48)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             648..670
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(701..720)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(702..720)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc
       61 cgcaagcttg tcgacggagc tcgaattctc atttatcttc ggtaatggga gagttgggga
      121 cagaggccgt ctgcaatgcg cgccatgttt caaggcgtgc ggcaagggcc gggttataca
      181 gtgcaagaat ggaggcggcc agcaaggcgg catttttggc gccggatgcc ccaatagcca
      241 aagtgcccac aggcacgccg ccgggcatct gcacaatgga aagcaggctg tccatgcctt
      301 taagagcgcg agattctact ggaacaccca gcacgggcag gcgtgtccat gccgcacaca
      361 tacccggcag atgtgcagct ccaccggccc cagcaataat cacattcagg ccgcgttcgg
      421 ctgccgtacg cgcataatcc gccaaccggt ctggcgtacg atgtgctaca acaatgaggg
      481 tttcatgcgg aatttccagc tctgtcagca atgcgtctgc atggcgcatg gtttcccagt
      541 ctgactggct gcccatgata atgcccacaa cgggggcaga ggctgcttta tcttctaaag
      601 cactggaagc agagggcagg ggggcggttt cgctcatcat atgtatatct ccttcttaaa
      661 gttaaacaaa attatttcta gagggaaacc gttgtggtct ccctatagtg agtcgtatta
      721 atttcgcggg atcgagatct cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt
      781 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga
      841 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc
      901 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc
      961 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtgga
//
