LOCUS       sequence-133942-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, Met15>Trp mutant (TdPurE2-M15W).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Kappock
  TITLE     Serine mutants of Treponema denticola PurE2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK696
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             586..603
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(656..674)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      670..717
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(841..860)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcgtcc gctggtgatt atccttatgg
       61 gatccagttc agattggggg catgccgaga aaattgctag cgaattgaaa acttttggta
      121 ttgagtatgc cattcgcatc ggctccgcgc ataaaacagc ggagcatgta gtgtctatgc
      181 tgaaagaata tgaagctttg gaccgtccta aactgtacat cacgatcgct gggcgtagta
      241 acgctttatc aggctttgtt gatggcttcg tcaaaggtgc aaccattgcc tgcccaccgc
      301 caagcgatag cttcgccggc gccgacattt attcttcgtt acggatgccg agcgggatta
      361 gtccggcact ggttcttgaa ccgaaaaatg cggcactcct ggcggctcgt attttttcac
      421 tgtacgataa agaaatcgca gattcggtca aatcctatat ggaatccaat gcgcagaaaa
      481 ttatcgaaga cgattcgaaa ctgaaacgct gaattcagcg tttcagtttc ttgtcgacgg
      541 agctcgaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac
      601 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      661 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      721 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
      781 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      841 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      901 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      961 a
//
