LOCUS       sequence-133943-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, Met15>Trp mutant (TdPurE2-M15W).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Kappock
  TITLE     Serine mutants of Treponema denticola PurE2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK696
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             613..635
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(666..685)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(667..685)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tcgagctccg tcgacaagaa actgaaacgc
      121 tgaattcagc gtttcagttt cgaatcgtct tcgataattt tctgcgcatt ggattccata
      181 taggatttga ccgaatctgc gatttcttta tcgtacagtg aaaaaatacg agccgccagg
      241 agtgccgcat ttttcggttc aagaaccagt gccggactaa tcccgctcgg catccgtaac
      301 gaagaataaa tgtcggcgcc ggcgaagcta tcgcttggcg gtgggcaggc aatggttgca
      361 cctttgacga agccatcaac aaagcctgat aaagcgttac tacgcccagc gatcgtgatg
      421 tacagtttag gacggtccaa agcttcatat tctttcagca tagacactac atgctccgct
      481 gttttatgcg cggagccgat gcgaatggca tactcaatac caaaagtttt caattcgcta
      541 gcaattttct cggcatgccc ccaatctgaa ctggatccca taaggataat caccagcgga
      601 cgcatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg aaaccgttgt
      661 ggtctcccta tagtgagtcg tattaatttc gcgggatcga gatctcgggc agcgttgggt
      721 cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg
      781 gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct
      841 gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt
      901 aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag
      961 gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac
     1021 cctga
//
