LOCUS       sequence-133949-        1133 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 
            tag and Trp404>Phe mutant (AarAH6-W404F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   TJ Kappock
  TITLE     AaCS exchange
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK241
FEATURES             Location/Qualifiers
     source          1..1133
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatgag cgcgtcgcag aaagaaggta
       61 agctatctac cgctaccatt tcggttgatg gaaaatccgc cgaaatgcct gtgctttcag
      121 gcactctggg accggatgtt atcgacatcc gcaaacttcc ggcgcaactg ggcgttttca
      181 cgttcgaccc aggttacggg gaaacagcgg cctgcaacag caaaatcacc tttattgatg
      241 gtgataaagg cgttctgctg caccgtggtt accctattgc gcagctggcc gaaaatgctt
      301 cctacgaaga agttatttat ctgcttttga atggcgaact gcccaacaag gcgcagtacg
      361 acaccttcac caacaccctt acaaaccata cgctgctgca cgagcagatc cgtaacttct
      421 ttaacggctt ccggcgtgat gcccacccaa tggccattct gtgtggtacg gttggggctt
      481 tgtctgcctt ctacccagat gccaacgata ttgccattcc cgccaatcgg gatctggccg
      541 ccatgcggct gattgccaaa atcccaacca ttgcggcatg ggcttacaaa tacacgcagg
      601 gtgaagcctt tatctacccg cggaatgatc tgaactacgc agaaaacttc ctgtccatga
      661 tgttcgcgcg catgtccgaa ccttacaagg tcaaccctgt tctggcccgc gccatgaacc
      721 ggattctgat tctgcatgcc gatcatgagc agaatgcctc tacctccacc gtacgtctgg
      781 ctggttctac aggggccaat ccgtttgcct gtattgctgc gggcattgcc gctctgtggg
      841 gacctgcaca tggtggcgca aacgaagctg tgctgaaaat gctggcccgt attggcaaga
      901 aagaaaatat tcctgccttt atcgcacagg tgaaggacaa gaacagcggc gtaaagctga
      961 tgggctttgg ccaccgcgtt tacaagaact tcgacccacg tgcgaagatc atgcagcaga
     1021 cctgccacga agtgctgaca gaacttggca ttaaggatga tccgctgctg gatctggcgg
     1081 ttgagctgga aaagattgct ctgagcgatg attacttcgt gcagcgcaaa ctt
//
