LOCUS       sequence-133953-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK279
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             652..674
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(705..724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(706..724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tctcatttat cttcggtaat gggagagttg
      121 gggacagagg ccgtctgcaa tgcgcgccat gtttcaaggc gtgcggcaag ggccgggtta
      181 tacagtgcaa gaatggaggc ggccagcaag gcggcatttt tggcgccgga tgccccaata
      241 gccaaagtgc ccacaggcac gccgccgggc atctgcacaa tggaaagcag gctgtccatg
      301 cctttaagag cgcgagattc tactggaaca cccagcacgg gcaggcgtgt ccatgccgca
      361 cacatacccg gcagatctgc agctccaccg gccccagcaa taatcacatt caggccgcgt
      421 tcggctgccg tacgcgcata atccgccaac cggtctggcg tacgatgtgc tgaaacaatg
      481 agggtttcat gcggaatttc cagctctgtc agcaatgcgt ctgcatggcg catggtttcc
      541 cagtctgact ggctgcccat gataatgccc acaacggggg cagaggctgc tttatcttct
      601 aaagcactgg aagcagaggg caggggggcg gtttcgctca tcatatgtat atctccttct
      661 taaagttaaa caaaattatt tctagaggga aaccgttgtg gtctccctat agtgagtcgt
      721 attaatttcg cgggatcgag atctcgggca gcgttgggtc ctggccacgg gtgcgcatga
      781 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
      841 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
      901 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
      961 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg
     1021 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgag
//
