LOCUS       sequence-133958-        1093 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gap-repair recombineering for Drosophila transgenesis, contains loxP
            cassette with GFP, chloramphenicol resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Haussmann IU, Ustaoglu P, Brauer U, Hemani Y, Dix TC, Soller M
  TITLE     Plasmid-based gap-repair recombineered transgenes reveal a central 
            role for introns in mutually exclusive alternative splicing in Down 
            Syndrome Cell Adhesion Molecule exon 4.
  JOURNAL   Nucleic Acids Res. 2018 Dec 12. pii: 5239037. doi: 
            10.1093/nar/gky1254.
  PUBMED    30541104
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 12. pii: 5239037. doi: 10.1093/nar/gky1254."
COMMENT     Sequence Label: pOT2 3GLA
FEATURES             Location/Qualifiers
     source          1..1093
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        108..126
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     108..125
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     134..153
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    254..323
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    complement(523..556)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(745..766)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgaattacaa cagtactgcg atgagtggca gggcggggcg taattggtac gtcgacgtta
       61 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      121 tatagaactc gagcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
      181 ataccgcacc gaatcgcgcg gaactaacga cagtcgctcc aaggtcgtca agccggtacg
      241 atgtaggtca ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg
      301 gcgcgtactc cacctcaccc atctggtcca tcatgatgaa cgggtcgagg tggcggtagt
      361 tgatcccggc gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg
      421 tggtcacggt gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg
      481 tcagcgggtt ctcgacggtc acggcgggca tctagtacgc gtataacttc gtatagcata
      541 cattatacga agttatatca agcttatcgc gtctaagttt aaatggcaag tgcatttttt
      601 catttttttt atttttctat ttgaggaaag ccgtaattga ctagattcga gataatatga
      661 attattaaat gggaaactct acaagcttgc aagatctgag tacttgtaca gctcgtccat
      721 gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
      781 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
      841 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      901 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
      961 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
     1021 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
     1081 cacctcggcg cgg
//
