LOCUS       sequence-133961-        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant 
            (AaPurE1wyf-H59N).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Kappock
  TITLE     Single Trp variants of Acetobacter aceti PurE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK666
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             629..646
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(699..717)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      713..760
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(884..903)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatga tgagcgaaac cgcccccctg
       61 ccctctgctt ccagtgcttt agaagataaa gcagcctctg cccccgttgt gggcattatc
      121 atgggcagcc agtcagactg ggaaaccatg cgccatgcag acgcattgct gacagagctg
      181 gaaattccgc atgaaaccct cattgtttca gcaaatcgta cgccagaccg gttggcggat
      241 tatgcgcgta cggcagccga acgcggcctg aatgtgatta ttgctggggc cggtggagct
      301 gcacatctgc cgggtatgtg tgcggcatat acacgcctgc ccgtgctggg tgttccagta
      361 gaatctcgcg ctcttaaagg catggacagc ctgctttcca ttgtgcagat gcccggcggc
      421 gtgcctgtgg gcactttggc tattggggca tccggcgcca aaaatgccgc cttgctggcc
      481 gcctccattc ttgcactgta taacccggcc cttgccgcac gccttgaaac atttcgcgca
      541 ttgcagacgg cctctgtccc caactctccc attaccgaag ataaatgaga attcgagctc
      601 cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc
      661 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      721 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      781 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      841 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      901 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      961 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaa
//
