LOCUS       sequence-133962-        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase, His59>Asn/Trp97>Tyr/Trp165>Phe mutant 
            (AaPurE1wyf-H59N).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Kappock
  TITLE     Single Trp variants of Acetobacter aceti PurE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK666
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             652..674
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(705..724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(706..724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
       61 cggccgcaag cttgtcgacg gagctcgaat tctcatttat cttcggtaat gggagagttg
      121 gggacagagg ccgtctgcaa tgcgcgaaat gtttcaaggc gtgcggcaag ggccgggtta
      181 tacagtgcaa gaatggaggc ggccagcaag gcggcatttt tggcgccgga tgccccaata
      241 gccaaagtgc ccacaggcac gccgccgggc atctgcacaa tggaaagcag gctgtccatg
      301 cctttaagag cgcgagattc tactggaaca cccagcacgg gcaggcgtgt atatgccgca
      361 cacatacccg gcagatgtgc agctccaccg gccccagcaa taatcacatt caggccgcgt
      421 tcggctgccg tacgcgcata atccgccaac cggtctggcg tacgatttgc tgaaacaatg
      481 agggtttcat gcggaatttc cagctctgtc agcaatgcgt ctgcatggcg catggtttcc
      541 cagtctgact ggctgcccat gataatgccc acaacggggg cagaggctgc tttatcttct
      601 aaagcactgg aagcagaggg caggggggcg gtttcgctca tcatatgtat atctccttct
      661 taaagttaaa caaaattatt tctagaggga aaccgttgtg gtctccctat agtgagtcgt
      721 attaatttcg cgggatcgag atctcgggca gcgttgggtc ctggccacgg gtgcgcatga
      781 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
      841 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
      901 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
      961 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg cta
//
