LOCUS       sequence-133964-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human RagA from a CMV promoter for stable expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Efeyan A, Schweitzer LD, Bilate AM, Chang S, Kirak O, Lamming DW, 
            Sabatini DM
  TITLE     RagA, but not RagB, is essential for embryonic development and adult
            mice.
  JOURNAL   Dev Cell. 2014 May 12;29(3):321-9. doi: 
            10.1016/j.devcel.2014.03.017. Epub 2014 Apr 24.
  PUBMED    24768164
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.03.017"; journalName: "Dev Cell"; date: 
            "2014-05-12- 12"; volume: "29"; issue: "3"; pages: "321-9"
COMMENT     Sequence Label: pLJM1 RagA
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..59
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccgtcagatc cgctagcgct accggtgcca ccatggacta caaggatgac gatgacaagg
       61 gtggtggtgg tggtgcgtcg acgatgccaa atacagccat gaagaaaaag gtgctgctga
      121 tggggaagag cgggtcgggg aagaccagca tgaggtcgat aatcttcgcc aattacattg
      181 ctcgcgacac ccggcgcctg ggggccacca ttgacgtgga acactcccac gtccgattcc
      241 tagggaacct ggtgctgaac ctgtgggact gtggcggtca ggacaccttc atggaaaatt
      301 acttcaccag ccagcgagac aatatcttcc gtaacgtgga agttttgatt tacgtgtttg
      361 acgtggagag ccgcgaactg gaaaaggaca tgcattatta ccagtcgtgt ctggaggcca
      421 tcctccagaa ctctcctgac gccaaaatct tctgcctggt gcacaaaatg gatctggttc
      481 aggaggatca gcgtgacctg atttttaaag agcgagagga agacctgagg cgtctgtctc
      541 gcccgctgga gtgtgcttgt tttcgaacgt ccatctggga tgagacgctc tacaaagcct
      601 ggtccagcat cgtctaccag ctgattccca acgttcagca gctggagatg aacctcagg
//
