LOCUS       sequence-133967-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gap-repair recombineering for Drosophila transgenesis, contains loxP
            cassette with GFP, Amp resistant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Haussmann IU, Ustaoglu P, Brauer U, Hemani Y, Dix TC, Soller M
  TITLE     Plasmid-based gap-repair recombineered transgenes reveal a central 
            role for introns in mutually exclusive alternative splicing in Down 
            Syndrome Cell Adhesion Molecule exon 4.
  JOURNAL   Nucleic Acids Res. 2018 Dec 12. pii: 5239037. doi: 
            10.1093/nar/gky1254.
  PUBMED    30541104
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 12. pii: 5239037. doi: 10.1093/nar/gky1254."
COMMENT     Sequence Label: pUC 3GLA
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     166..187
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    376..409
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    609..678
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(779..798)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     898..920
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(958..976)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
       61 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      121 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      181 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt actcagatct
      241 tgcaagcttg tagagtttcc catttaataa ttcatattat ctcgaatcta gtcaattacg
      301 gctttcctca aatagaaaaa taaaaaaaat gaaaaaatgc acttgccatt taaacttaga
      361 cgcgataagc ttgatataac ttcgtataat gtatgctata cgaagttata cgcgtactag
      421 atgcccgccg tgaccgtcga gaacccgctg acgctgcccc gcgtatccgc acccgccgac
      481 gccgtcgcac gtcccgtgct caccgtgacc accgcgccca gcggtttcga gggcgagggc
      541 ttcccggtgc gccgcgcgtt cgccgggatc aactaccgcc acctcgaccc gttcatcatg
      601 atggaccaga tgggtgaggt ggagtacgcg cccggggagc ccaagggcac gccctggcac
      661 ccgcaccgcg gcttcgagac ctgacctaca tcgtaccggc ttgacgacct tggagcgact
      721 gtcgttagtt ccgcgcgatt cggtgcggta tttcacaccg catatggtgc actctcagta
      781 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
      841 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
      901 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
      961 tcgtgatacg cctattttt
//
