LOCUS       sequence-133970-        1142 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 
            tag and Asp371>Asn mutant (AarAH6-D371N).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   TJ Kappock
  TITLE     AaCS exchange
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK699
FEATURES             Location/Qualifiers
     source          1..1142
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgaggcg
       61 tttggcaagc ggcacatagt cacgctgcgg tgcgccaata taaagctggc gagggcggct
      121 gatacgctgg cccggttctt caatcatttc cttccactgg ctcacccagc cggtggtgcg
      181 ggctacggca aacagcacag taaacatact ggtggggatg cccatggcct tgagaatgat
      241 gccagagtag aaattcacat tcgggtaaag tttgcgctgc acgaagtaat catcgctcag
      301 agcaatcttt tccagctcaa ccgccagatc cagcagcgga tcatccttaa tgccaagttc
      361 tgtcagcact tcgtggcagg tctgctgcat gatcttcgca cgtgggtcga agttcttgta
      421 aacgcggtgg ccaaagccca tcagctttac gccgctgttc ttgtccttca cctgtgcgat
      481 aaaggcagga atattttctt tcttgccaat acgggccagc attttcagca cagcttcgtt
      541 tgcgccacca tgtgcaggtc cccacagagc ggcaatgccc gcagcaatac aggcaaacgg
      601 attggcccct gtagaaccag ccagacgtac ggtggaggta gaggcattct gctcatgatc
      661 ggcatgcaga atcagaatcc ggttcatggc gcgggccaga acagggttga ccttgtaagg
      721 ttcggacatg cgcgcgaaca tcatggacag gaagttttct gcgtagttca gatcattccg
      781 cgggtagata aaggcttcac cctgcgtgta tttgtaagcc catgccgcaa tggttgggat
      841 tttggcaatc agccgcatgg cggccagatc ccgattggcg ggaatggcaa tatcgttggc
      901 atctgggtag aaggcagaca aagccccaac cgtaccacac agaatggcca ttgggtgggc
      961 atcacgccgg aagccgttaa agaagttacg gatctgctcg tgcagcagcg tatggtttgt
     1021 aagggtgttg gtgaaggtgt cgtactgcgc cttgttgggc agttcgccat tcaaaagcag
     1081 ataaataact tcttcgtagg aagcattttc ggccagctgc gcaatagggt aaccacggtg
     1141 ca
//
