LOCUS       sequence-133973-        1157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 
            tag and Trp400>Phe mutant (AarAH6-W400F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   TJ Kappock
  TITLE     AaCS exchange
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK708
FEATURES             Location/Qualifiers
     source          1..1157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(35..52)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgaggc
       61 gtttggcaag cggcacatag tcacgctgcg gtgcgccaat ataaagctgg cgagggcggc
      121 tgatacgctg gcccggttct tcaatcattt ccttccactg gctcacgaag ccggtggtgc
      181 gggctacggc aaacagcaca gtaaacatac tggtggggat gcccatggcc ttgagaatga
      241 tgccagagta gaaatccaca ttcgggtaaa gtttgcgctg cacgaagtaa tcatcgctca
      301 gagcaatctt ttccagctca accgccagat ccagcagcgg atcatcctta atgccaagtt
      361 ctgtcagcac ttcgtggcag gtctgctgca tgatcttcgc acgtgggtcg aagttcttgt
      421 aaacgcggtg gccaaagccc atcagcttta cgccgctgtt cttgtccttc acctgtgcga
      481 taaaggcagg aatattttct ttcttgccaa tacgggccag cattttcagc acagcttcgt
      541 ttgcgccacc atgtgcaggt ccccacagag cggcaatgcc cgcagcaata caggcaaacg
      601 gattggcccc tgtagaacca gccagacgta cggtggaggt agaggcattc tgctcatgat
      661 cggcatgcag aatcagaatc cggttcatgg cgcgggccag aacagggttg accttgtaag
      721 gttcggacat gcgcgcgaac atcatggaca ggaagttttc tgcgtagttc agatcattcc
      781 gcgggtagat aaaggcttca ccctgcgtgt atttgtaagc ccatgccgca atggttggga
      841 ttttggcaat cagccgcatg gcggccagat cccgattggc gggaatggca atatcgttgg
      901 catctgggta gaaggcagac aaagccccaa ccgtaccaca cagaatggcc attgggtggg
      961 catcacgccg gaagccgtta aagaagttac ggatctgctc gtgcagcagc gtatggtttg
     1021 taagggtgtt ggtgaaggtg tcgtactgcg ccttgttggg cagttcgcca ttcaaaagca
     1081 gataaataac ttcttcgtag gaagcatttt cggccagctg cgcaataggg taaccacggt
     1141 gcagcagaac gccttta
//
