LOCUS       sequence-133981-        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Complementation of NME1 gene disruption or mutation in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Schmitt ME, Clayton DA
  TITLE     Nuclear RNase MRP is required for correct processing of pre-5.8S 
            rRNA in Saccharomyces cerevisiae.
  JOURNAL   Mol Cell Biol. 1993 Dec;13(12):7935-41.
  PUBMED    8247008
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1993-12"; volume: "13(12)"; pages: "7935-41"
COMMENT     Sequence Label: pMES140
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(501..520)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     620..642
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(680..698)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgcttaaaaa gattctcttt ttttatgata tttgtacata aactttataa atgaaattca
       61 taatagaaac gacacgaaat tacaaaatgg aatatgttca tagggtagac gaaactatat
      121 acgcaatcta catacattta tcaagaagga gaaaaaggag gatgtaaagg aatacaggta
      181 agcaaattga tactaatggc tcaacgtgat aaggaaaaag aattgcactt taacattaat
      241 attgacaagg aggagggcac cacacaaaaa gttaggtgta acagaaaatc atgaaactat
      301 gattcctaat ttatatattg gaggattttc tctaaaaaaa aaaaaataca acaaataaaa
      361 aacactcaat gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc
      421 cataatggtg aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag
      481 tcgatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
      541 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      601 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
      661 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      721 ataatggttt cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa
      781 tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat
      841 tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa
      901 ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc
      961 agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg
     1021 tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa
     1081 gagcaagata aaaggtagta tttgttgg
//
