LOCUS       sequence-133999-        4411 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TCR beta chain transgene expression plasmid which has a unique ClaI 
            site for the insertion of a TCR beta chain VDJ exon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Barnden MJ, Allison J, Heath WR, Carbone FR
  TITLE     Defective TCR expression in transgenic mice constructed using 
            cDNA-based alpha- and beta-chain genes under the control of 
            heterologous regulatory elements.
  JOURNAL   Immunol Cell Biol. 1998 Feb;76(1):34-40.
  PUBMED    9553774
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunol 
            Cell Biol."; date: "1998-02"; volume: "76(1)"; pages: "34-40"
COMMENT     Sequence Label: p3A9cbetaTCR
FEATURES             Location/Qualifiers
     source          1..4411
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      62..71
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tcacgcccca ggaagtgtaa tgaggaagat tgagaacact tcaacctttt cgtacttcta
       61 agccaccatg gctacaaggc tcctctggta cacagtactt tgtctcctgg gtgcaggtag
      121 gtcctaagga caaaaagaca gtctttgact tggagattgc caacccatat ctaagtatcg
      181 atagtaggag acagctctca acttcaccct ttctggggga gcgggatgaa aagggattgg
      241 gtgccaaacg ttgttcgctc tctcgcaagc caggacaagg taggtttctt tctcttttct
      301 ctgaggtatg catgcactgg gtgggtggtg aagaaaaaga attaattagg actctggaag
      361 caaacttggt tttgaaaata aatagccggc acaaagaact aaagggcaga taaaagcagg
      421 gagattccaa gaggtggtgc tgggaaatct agagttccca gtggcaaaag tgggagaggg
      481 tcttttgaaa ggagcgaaat gagtaaagca agaaaccaat gacagacata tataaactag
      541 aacagaagaa tcaaaagtct ggtggtgttg actcgcgatt aaattccatc cactgttggc
      601 caagtttggg atcacttgaa gcagtgttgg agacccagta ttaaaacaca ttctcacaac
      661 tgattggccc caagcagggg gagcttagtc actggggtct ggtaatcctc aggttatttg
      721 cctaaatcct gacctgaaat cttcactgct caggctagac agacaggcta tatcatgaag
      781 ggatttcatt gaccacacgc acacattaaa atagaaacat ttatctcggg gaaaatgctt
      841 gaaacccctt aggatcccag tattagaatg gactttaaat atcgcgtggc agtggggggt
      901 caaaggtata gaagagaaat aattcacagc ctggatatgt gaacctgtat tatttggtaa
      961 ccatagcaac agtgaacatc atttgtggtt ggaacattaa gcgggatcca agagaactca
     1021 gccatgttaa attgtgtcta aaggaaatgc tgggcctctg tagacaggtg gaataaagaa
     1081 aagagccaga ttgtatagcc agatgaggct gaattccagt cctggctctt tgatgataag
     1141 gtaagcttga gtatgttaat gtgtgaccta gttctatggc tacagatagt gaggtagttt
     1201 gaaaagagga cgatgagttt ttcaatttta ctcacagcta gtgcagttct caagcaaatc
     1261 acataccagc agcagcttac taaagcacaa ttcattttca caagcaatta taccgcccaa
     1321 caatcctcag ggattcacac acatggatat ttagctgccc cccccccccc ccgttttccc
     1381 attgaattat acaagtaata cttcagggtg tagtgagaat ctgcttctat ttgtcctgac
     1441 agtggtgttg attaatacaa tagaaaacaa aatgcctgac tcaggcaagt gtttgatttt
     1501 tcacaacgtg agctaaatca ttgcttaagt gagccgggtc agagaagtgc atcccagagt
     1561 tcccatggaa agctgggaga atctcaaagc caatatctct gggacacagt aagactagac
     1621 agacctaaag cagagtagat agagtcaagt accacggagg gaggcaagaa tggtactctt
     1681 tctatctagg tgcagcctgt aaagacctag gtgcattgaa aatgatgcac tgggacaaag
     1741 aaactgatca gaggccagat gtgctaggcc cacatctaca ctcccaacac tgggaagact
     1801 taaacaagag cagtgccagg agtgtgaggc caaccggagc tacacaaaga gttctaagcc
     1861 agtgtggaat atagagaaat ttaggccatt atggattgca gattaagatc ctgtccaccc
     1921 caccccctaa aaatccaaag aaagaaaaga aaactataga gactcctacc aaaaagaatc
     1981 agaattccag catgatggcc gaagagtttg aagccaagca ctaagcttgt gatcttgtat
     2041 aaggtctgtg caggttcttt taccgttgtt tcaagaccag aagtaaagcg atatccacag
     2101 aaataggaga aaggatcaaa ataaagtttc tccattatcc agaaggccgt gggttgctaa
     2161 cacaaaatat atctatctct ttatcttcag caaatcactt ggaagcaaag aacagagaga
     2221 aagtcacaaa gaactgggaa tgtttagagg cagagaagtt gataggttta tttcacacac
     2281 cacaaaactc agtaaatctt acatactcat gaatccactg tagaccagga tgtctacaca
     2341 tgcaggaatg aaaatgggcc atctattgta gtctgaagaa agccttccca tgccaaatgt
     2401 ttatctcaac tttaattacc ctttaatcct ggcctcttag cttccataac ttggctgtgg
     2461 caagatctta taaatccttc tgtctcttct ggggaagtca gtgtttcaca tttcactttg
     2521 gaaagaaatt gatgcagaga cattaaagag actctcatgg tcacacgggc acagaaggtc
     2581 attgctcaga tttagattct gtagttcaga tttctgtggc aggctctaat taaatatctg
     2641 cagaatatag taagaaataa gcgtgtcgtg atagagcagg atcaaaacta tgtcaacaaa
     2701 ggacatagtt ttacgtttcc taaagaacac tgggtcagac catttgttgt atctccttat
     2761 gagaatggat gaatataaat gacaagcaaa gtgaaagatt aatgccacag agaaatggaa
     2821 tggtgattcc aaagccaaca ggacctgctt gctttttcag aaactctctc agccctgaac
     2881 ctttccaaat acaaatgatt tgtacctgat ccagacagtt atcagcaaaa tactaattcc
     2941 acctctcttt actttccaga ggatctgaga aatgtgactc cacccaaggt ctccttgttt
     3001 gagccatcaa aagcagagat tgcaaacaaa caaaaggcta ccctcgtgtg cttggccagg
     3061 ggcttcttcc ctgaccacgt ggagctgagc tggtgggtga atggcaagga ggtccacagt
     3121 ggggtcagca cggaccctca ggcctacaag gagagcaatt atagctactg cctgagcagc
     3181 cgcctgaggg tctctgctac cttctggcac aatcctcgaa accacttccg ctgccaagtg
     3241 cagttccatg ggctttcaga ggaggacaag tggccagagg gctcacccaa acctgtcaca
     3301 cagaacatca gtgcagaggc ctggggccga gcaggtaagt gcggagctca tgaggaaagt
     3361 aaacagcact agtacttcaa aaaatatgaa acaatccatg tagaagtgaa gaatagaccc
     3421 aggaaaaggc cagagtggtg ggacagatga tcaagctcat ggtgtcagaa aaccatagcc
     3481 tatgcttcct cccaaggagt atgtatgtaa actcagtggg gcagctcagg ccaattggct
     3541 tcccagttct ttagtgtctc agagctgtgc ttaagagttc tcccatacct ccctcacaac
     3601 ctagcatcgc tcatccccat ccctgctgct agatttttaa ggtcactctg acagtgtctt
     3661 ataactttcc cagcaccatc agaaagacag tgtgaggact ataagaggag agtgcttaca
     3721 ccatctgaca tgcatgtgtc tgtggccttt acattcggct ttaagttttg ttgttgggtg
     3781 ttcaatgtcc cccaaagtgg ctttctttca cccaattttc tccctctcct ttctttcaga
     3841 ctgtggaatc acttcaggtg agtagatctt ccgactttct ctacgtcttc tgtggtcttg
     3901 gctttgaaaa caggacacaa atatcctata gacatgaggg tcggggctgc ccagaggaac
     3961 tagctcacaa acacctacta actctccttt cctgtcaaca gcatcctatc atcagggggt
     4021 tctgtctgca accatcctct atgagatcct actggggaag gccaccctat atgctgtgct
     4081 ggtcagtggc ctggtgctga tggccatggt aagaatggta ggatggacaa atggttggag
     4141 gtatagactt cagtgtatgg atataaaggg atctcagagg aggacccagc cctgatatcc
     4201 tgccatttca aaaaagacca taaaaaacac agtgcaaagc aaaaacacag gaatgcttat
     4261 gtttgtactc ctggaagatg aagagaacca aggagctctc ttcaagatca tacttgaaaa
     4321 tctccttttt gtatcccctt ccctctgctc catggattct ggaggtctaa caatgtcttc
     4381 tctttctcag gtcaagaaaa aaaattcctg a
//
