LOCUS       sequence-134005-        5985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains membrane bound form of ovalbumin (OVA) cDNA in pBlueRIP 
            (used to generate RIP-mOVA mice).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Kurts C, Heath WR, Carbone FR, Allison J, Miller JF, Kosaka H
  TITLE     Constitutive class I-restricted exogenous presentation of self 
            antigens in vivo.
  JOURNAL   J Exp Med. 1996 Sep 1;184(3):923-30.
  PUBMED    9064352
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "1996-09-1"; volume: "184(3)"; pages: "923-30"
COMMENT     Sequence Label: pBlueRIP.TfrOVA
FEATURES             Location/Qualifiers
     source          1..5985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..132
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(133..201,202..993)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAISMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(351..370)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1164..1752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1906..1923
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2076..2081,2082..2099,2100..2106)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2114..2130
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2119..2141
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2138..2154
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2138..2154
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2175..2193
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2221..2237
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2222..2238
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3765..3788
                     /codon_start=1
                     /product="antigenic CD8+ T cell epitope from chicken 
                     ovalbumin"
                     /label=OVA peptide
                     /translation="SIINFEKL"
     intron          4942..5007
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    5167..5301
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5204..5223)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5258..5277
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5298..5314)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5298..5314)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5346..5365)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5347..5365)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5372..5389)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5372..5388)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5381..5403)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5530..5985
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5617..5636)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5827..5848
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
       61 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      121 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      181 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      241 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
      301 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      361 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      421 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      481 gtaagagaat tatgcagtgc tgccataagc atgagtgata acactgcggc caacttactt
      541 ctgacaacga tcggaggacc gaaggagcta accgcttttt ttcacaacat gggggatcat
      601 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      661 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      721 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
      781 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
      841 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
      901 gtagttatct acacgacggg cagtcaggca actatggatg aacgaaatag acagatcgct
      961 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1021 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1081 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1261 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     1321 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1381 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1441 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1501 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     1561 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1621 ggaacaggag agcgcacgag ggagcttcca ggggggaacg cctggtatct ttatagtcct
     1681 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggccg
     1741 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1801 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1861 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1921 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     1981 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2041 aatgtgagtt acctcactca ttaggcaccc caggctttac actttatgct tccggctcct
     2101 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     2161 tacgccaagc tcggaattaa ccctcactaa agggaacaaa agctggtacc gggccccccc
     2221 tcgaggtcga cggtatcgat aagcagcttg atatcgaatt cctgcagccc gggggatccc
     2281 ccaaccactc caagtggagg ctgagaaagg ttttgtagct gggtagagta tgtactaaga
     2341 gatggagaca gctggctctg agctctgaag caagcacctc ttatggagag ttgctgacct
     2401 tcaggtgcaa atctaagata ctacaggaga atacaccatg gggcttcagc ccagttgact
     2461 cccgagtggg ctatgggttt gtggaaggag agatagaaga gaagggacct ttcttcttga
     2521 attctgcttt ccttctacct ctgagggtga gctggggtct cagctgaggt gaggacacag
     2581 ctatcagtgg gaactgtgaa acaacagttc aagggacaaa gttactaggt cccccaacaa
     2641 ctgcagcctc ctggggaatg atgtggaaaa atgctcagcc aaggacaaag aaggcctcac
     2701 cctctctgag acaatgtccc ctgctgtgaa ctggttcatc aggccaccca ggagccccta
     2761 ttaagactct aattacccta aggctaagta gaggtgttgt tgtccaatga gcactttctg
     2821 cagacctagc accaggcaag tgtttggaaa ctgcagcttc agcccctctg gccatctgct
     2881 gatccaccct taatgggaca aacagcaaag tccaggggtc aggggggggg tgctttggac
     2941 tataaagcta gtggggattc agtaaccccc agccctaact ctagcaagct tgaattccat
     3001 cgggtggcgg ctcgggacgg aggacgcgct agtgttcttc tgtgtggcag ttcagaatga
     3061 tggatcaagc tagatcagca ttctctaact tgtttggtgg agaaccattg tcatataccc
     3121 ggttcagcct ggctcggcaa gtagatggcg ataacagtca tgtggagatg aaacttgctg
     3181 tagatgaaga agaaaatgct gacaataaca caaaggccaa tgtcacaaaa ccaaaaaggt
     3241 gtagtggaag tatctgctat gggactattg ctgtgatcgt ctttttcttg attggattta
     3301 tgattggcta cttgggctat tgtaaagggg tagaaccaaa aactgagtgt gagagactgg
     3361 caggaaccga gtctccagtg agggaggagc caggagagga cttccctgca gatcaagcca
     3421 gagagctcat caattcctgg gtagaaagtc agacaaatgg aattatcaga aatgtccttc
     3481 agccaagctc cgtggattct caaactgcaa tggttctggt taatgccatt gtcttcaaag
     3541 gactgtggga gaaaacattt aaggatgaag acacacaagc aatgcctttc agagtgactg
     3601 agcaagaaag caaacctgtg cagatgatgt accagattgg tttatttaga gtggcatcaa
     3661 tggcttctga gaaaatgaag atcctggagc ttccatttgc cagtgggaca atgagcatgt
     3721 tggtgctgtt gcctgatgaa gtctcaggcc ttgagcagct tgagagtata atcaactttg
     3781 aaaaactgac tgaatggacc agttctaatg ttatggaaga gaggaagatc aaagtgtact
     3841 tacctcgcat gaagatggag gaaaaataca acctcacatc tgtcttaatg gctatgggca
     3901 ttactgacgt gtttagctct tcagccaatc tgtctggcat ctcctcagca gagagcctga
     3961 agatatctca agctgtccat gcagcacatg cagaaatcaa tgaagcaggc agagaggtgg
     4021 tagggtcagc agaggctgga gtggatgctg caagcgtctc tgaagaattt agggctgacc
     4081 atccattcct cttctgtatc aagcacatcg caaccaacgc cgttctcttc tttggcagat
     4141 gtgtttcccc ttaaaagaag aaagctgaaa aactctgtcc cttccaacaa gacccagagc
     4201 actgtagtat caggggtaaa atgaaaagta tgttatctgc tgcatccaga cttcataaaa
     4261 gctggagctt aatctagaaa aaaaatcaga aagaaattac actgtgagaa caggtgcaat
     4321 tcacttttcc tttacacaga gtaatactgg taactcatgg atgaaggctt aagggaatga
     4381 aattggactc acagtactga gtcatcacac tgaaaaatgc aacctgatac atcagcagaa
     4441 ggtttatggg ggaaaaatgc agccttccaa ttaagccaga tatctgtatg accaagctgc
     4501 tccagaatta gtcactcaaa atctctcaga ttaaattatc aactgtcacc aaccattcct
     4561 atgctgacaa ggcaattgct tgttctctgt gttcctgata ctacaaggct cttcctgact
     4621 tcctaaagat gcattataaa aatcttataa ttcacatttc tccctaaact ttgactcaat
     4681 catggtatgt tggcaaatat ggtatattac tattcaaatt gttttccttg tacccatatg
     4741 taatgggtct tgtgaatgtg ctcttttgtt cctttaatca taataaaaac atgtttaagc
     4801 aaaaaaaaaa aaaaaaaaaa aaaaaaaagg aattcgccca tgggcgcccc agatccaagc
     4861 ttgggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac caaactacct
     4921 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     4981 ctgattctaa ttgtttgtgt attttagatt cacagtccca aggctcattt caggcccctc
     5041 agtcctcaca gtctgttcat gatcataatc agccatacca catttgtaga ggttttactt
     5101 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     5161 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5221 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5281 gtatcttatc atgtctggat ccactagttc tagagcggcc gccaccgcgg tggagctcca
     5341 attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa cgtcgtgact
     5401 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatcccccc ttcgccagct
     5461 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgt agcctgaatg
     5521 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5581 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5641 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5701 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5761 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5821 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5881 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5941 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattt
//
