LOCUS       sequence-134007-        1902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mouse Drp1 isoform b (minus residues 
            514-602) with C-terminal His-tag and PreScission protease cleavage 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Liu R, Chan DC
  TITLE     The mitochondrial fission receptor Mff selectively recruits 
            oligomerized Drp1.
  JOURNAL   Mol Biol Cell. 2015 Dec 1;26(24):4466-77. doi: 
            10.1091/mbc.E15-08-0591. Epub 2015 Oct 7.
  PUBMED    26446846
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E15-08-0591"; journalName: "Mol Biol Cell"; date: 
            "2015-12-1- 1"; volume: "26"; issue: "24"; pages: "4466-77"
COMMENT     Sequence Label: pET21-Drp1(deltaIB)
FEATURES             Location/Qualifiers
     source          1..1902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1840..1863
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1885..1902
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggaggcgc tgatcccggt catcaataag ctgcaggacg tcttcaacac agtgggtgcg
       61 gacatcatcc agctgcctca gatcgtcgta gtgggaacgc agagcagtgg gaagagctca
      121 gtgctggaaa gcctagtggg cagggacctt cttcccagag gaactggtgt ggtcacccgg
      181 agacctctca ttctgcagct agtccacgtt tcaccagaag ataaaagaaa aacaacagga
      241 gaagaaaatg gagttgaagc agaagaatgg ggtaaatttc ttcacaccaa aaacaagctt
      301 tacacagatt ttgatgaaat tcgacaagaa attgaaaatg aaactgaaag aatttcagga
      361 aataataagg gggtaagccc tgagccaatc catctcaagg ttttctcgcc caacgttgtc
      421 aacctgacac ttgtggattt accgggaatg accaaagtac ctgtaggcga tcagcccaag
      481 gacatcgagc ttcagatcag agaacttatt cttcggttca tcagtaatcc caattccatt
      541 atcctcgccg tcactgctgc aaatacagat atggcaacat cagaagcact caagatttcc
      601 agagaggtag atccagatgg gcgcagaact ctagctgtaa tcactaaact tgatctcatg
      661 gatgcgggta ctgatgccat ggatgtattg atgggaaggg ttattccagt caagcttgga
      721 ataattggag tagttaacag aagccaactg gatattaaca ataagaagag tgtaactgat
      781 tcaatccgtg atgagtatgc ttttcttcaa aagaagtacc catctctggc caacagaaat
      841 ggaacaaagt atcttgctag gaccctgaat aggttactta tgcatcatat cagagattgt
      901 ttaccagagc tgaaaacaag aataaatgtc ttagctgctc agtatcagtc tcttctaaat
      961 agctatggtg aaccggtgga tgataaaagt gctactttac tccagcttat taccaaattt
     1021 gccacagagt attgtaacac gattgaagga accgcaaagt acattgaaac ttctgagcta
     1081 tgcggtggtg ctaggatttg ttatattttc catgagactt tcgggcgaac cttagaatct
     1141 gtggacccac taggtggcct taacactatt gacatcctga ctgccatcag aaatgctact
     1201 ggcccccgtc ctgctttatt tgtgcctgaa gtttcatttg agttactggt caaacgtcag
     1261 attaagcgtc tagaagagcc cagcctacgg tgtgtggagc tggtccatga ggagatgcag
     1321 aggatcattc agcattgtag caattacagc acacaggaat tgttacggtt ccctaaactt
     1381 cacgatgcca tagttgaagt agtgacctgt cttcttcgta aaaggttgcc cgtgacaaat
     1441 gaaatggtgc ataacttagt ggcaattgag ctagcgtata tcaacacaaa acaccccgac
     1501 tttgctgatg cctgtgggct aatgaacaat aatatagagg aacagcgaga ttgtgaggtt
     1561 attgaaagac ttatcaaatc atattttcta attgtcagaa agaatattca agacagtgtc
     1621 ccaaaggcag taatgcattt tttggttaat catgtgaaag atactcttca gagtgaactg
     1681 gtagggcagc tgtataagtc atccttatta gatgaccttc tgactgaatc cgaggacatg
     1741 gcccaacgaa gaaaagaagc agcggatatg ctgaaggcat tacaaggagc cagtcaaatt
     1801 attgctgaaa tccgagagac tcatctttgg gcggatccgc tggaagttct gttccagggg
     1861 cccaagcttg cggccgcact cgagcaccac caccaccacc ac
//
