LOCUS       sequence-134012-        3149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces human phosphoribosyl pyrophosphate amidotransferase (PRAT),
            synthetic gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Kappock
  TITLE     Iron-sulfur cluster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK694
FEATURES             Location/Qualifiers
     source          1..3149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        27..670
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     564..587
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     676..692
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     676..692
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     RBS             1923..1931
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(2256..2272)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2257..2273)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     terminator      2275..2522
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2291..2309)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(2540..2559)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2541..2559)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2569..2586)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2569..2585)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2578..2600)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2726..3107
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2813..2832)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3023..3044
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gggaacaaaa gctggagctc agtttatcat tatcaatact cgccatttca aagaatacgt
       61 aaataattaa tagtagtgat tttcctaact ttatttagtc aaaaaattag ccttttaatt
      121 ctgctgtaac ccgtacatgc ccaaaatagg gggcgggtta cacagaatat ataacattgt
      181 aggtgtctgg gtgaacagtt tattcctggc atccactaaa tataatggag cccgcttttt
      241 aagctggcat ccagaaaaaa aaagaatccc agcaccaaaa tattgttttc ttcaccaacc
      301 atcagttcat aggtccattc tcttagcgca actacagaga acaggggcac aaacaggcaa
      361 aaaacgggca caacctcaat ggagtgatgc aacctgcctg gagtaaatga tgacacaagg
      421 caattgaccc acgcatgtat ctatctcatt ttcttacacc ttctattacc ttctgctctc
      481 tctgatttgg aaaaagctga aaaaaaaggt tgaaaccagt tccctgaaat tattccccta
      541 cttgactaat aagtatataa agacggtagg tattgattgt aattctgtaa atctatttct
      601 taaacttctt aaattctact tttatagtta gtcttttttt tagttttaaa acaccagaac
      661 ttagtttcga cggattctag aactagtgga tccatggaat tggaagaatt gggtattaga
      721 gaagaatgtg gtgtctttgg ttgtattgct tctggtgaat ggccaaccca attggacgtc
      781 ccacatgtca tcaccttggg tttggttggt ttacaacaca gaggtcaaga gtctgccggt
      841 attgttactt ctgacggttc ctccgtccca actttcaagt ctcacaaggg tatgggttta
      901 gttaaccacg tcttcactga ggataatttg aagaaattat acgtttccaa cttgggtatt
      961 ggtcacactc gttatgctac tactggtaaa tgcgaattgg aaaactgtca accattcgtt
     1021 gttgaaactt tgcacggtaa gattgccgtt gctcacaacg gtgaattggt caacgccgct
     1081 agattgcgta agaagttgtt gagacatggt attggtttgt ctacttcttc tgactctgaa
     1141 atgatcaccc aattattggc ctacactcca ccacaagaac aagacgacac cccagactgg
     1201 gtcgcccgta tcaagaactt aatgaaggaa gccccaactg cttactcctt gttgattatg
     1261 caccgtgatg tcatctacgc tgttagagat ccatacggta acagaccatt atgtatcggt
     1321 agattgattc ctgtttccga cattaacgac aaggaaaaaa agacctctga aactgaaggt
     1381 tgggttgttt cttccgaatc ttgctctttt ttatctattg gtgccagata ttatagagaa
     1441 gttttgcctg gtgaaatcgt tgaaatttct agacacaacg ttcaaacttt ggacatcatc
     1501 tccagatctg aaggtaaccc agtcgctttc tgcatcttcg aatacgttta ctttgccaga
     1561 cctgactcca tgttcgaaga tcaaatggtt tataccgtca gatacagatg tggtcaacaa
     1621 ttggctatcg aagctccagt cgacgctgac ttggtttcta ctgtccctga atccgctacc
     1681 ccagccgctt tagcttacgc tggtaagtgt ggtttgccat acgtcgaagt tttgtgtaag
     1741 aacagatatg ttggtagaac tttcattcaa ccaaatatga gattgcgtca attgggtatc
     1801 gctaaaaagt tcggtgtttt gtccgacaac ttcaagggta agagaattgt tttagtcgac
     1861 gattccattg ttcgtggtaa caccatctct ccaattatta agttgttgaa ggaatctggt
     1921 gctaaggagg tccatattag agttgcttct ccaccaatca agtacccatg tttcatgggt
     1981 atcaacattc caactaagga ggaattaatc gccaacaaac cagagttcga tcacttagct
     2041 gaatacttag gtgctaactc cgttgtttac ttatctgttg aaggtttggt ttcttctgtt
     2101 caagaaggta tcaagttcaa gaagcaaaag gaaaagaagc acgacatcat gatccaagaa
     2161 aatggtaacg gtttggaatg tttcgaaaag tccggtcatt gtactgcttg tttgactggt
     2221 aagtacccag ttgaattgga atggtaaaag cttatcgata ccgtcgacct cgagtcatgt
     2281 aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg
     2341 aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat
     2401 taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat
     2461 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt
     2521 gcggccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     2581 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     2641 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2701 gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2761 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2821 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2881 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2941 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3001 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaaacaa cactcaaccc
     3061 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     3121 aaatgagctg atttaacaaa aatttaacg
//
