LOCUS       sequence-134025-       13374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13374)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 13374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB332
FEATURES             Location/Qualifiers
     source          1..13374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1532..1656
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1694..1699,1700..1717,1718..1724)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1778..2437
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1844..1863)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2779..3084
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2963..2982
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3125..3249)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3281..3533
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3567..3583)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3567..3583)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3580..3602)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3591..3607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3615..3621,3622..3639,3640..3645))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3660..3681
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3766..3941
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6025..6701)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6068..6088)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6896..6920
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7441..8232
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8478..9066
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8967..8986
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9220..9237
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9252..9392
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9318..9337)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9478..9500
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9736..9930
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9996..11069)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11498..12127)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagaccagc cattatagca actatgttaa aagtgacagt tttctctaat
      361 cgggaatctt gtccgtattg aaagttccct gttcagtgac tgagccgctt aataatattt
      421 tcagtttcga gtacgaggga gagattgaac aaattttggg gtttcagggc taaattctgc
      481 aggggcttta gttttgagtc gggtgaaact ttcctaagtc ttggcaatga actcacattc
      541 gccattgtac ctattaaatg acccaaacat ccttgacaac aaattgacaa gtacgggaac
      601 atgatcttca atatgaaatt taatatgtcc tttatgttgg ctttcctcat caatgattgg
      661 aaactcacat acaaagaaaa gcgtgatata ataaatcact agaatcttct ccgctttcta
      721 agacagtaat gatggatcca cgtgagtgtg aaacatcaat atcaacatcg gtaggagtag
      781 agaagtttct aaatcagaaa aagtgaatag acaagacaac ttactccttg aacgagaata
      841 ttcctcgaga aagagggaaa agagtaaaaa gagtaaaaag ccatggtaca gaatagccca
      901 ttccatggtt attgcagtca aaccgctacg ccagcaaaaa cggaacgaaa ctaaacccgt
      961 tccacaaatt gcgaaagatg gaatgtattc gccatgtata tttcgagatt attatgagac
     1021 ttccggtccc gggaagatgg cctgggttca ccgggatcag ccgaattccg acgtgatgga
     1081 gcacggccat gcatacttct agagaagtcg gccaccgtga aattcgacga cgagatggta
     1141 ctagaatcac cgggacagta cacacctcgt tcatggcctc gagcattcga tgtcgctctc
     1201 acctgcacag cctcgaggta tcgagagggt tctaggatgg accagcgatg tctcgaggaa
     1261 tttagaaaga tttcatgcgc tcgttgtgtg agagccaagg aagcacatta tggggcctat
     1321 aagtaccacg caagggcctg gggttcccat caatcaaatc agtgttcaga cgatttcctc
     1381 aagcgatcat tgcaccgtga tcagtgaagc acagacttca agcttttggc ttgcctactt
     1441 ccacgttccc atccgagatc gacaccgaat tgttgagttc tgtctgatct tcctgcagtc
     1501 gagagcgaag ttccaccatc tagagttatc aacaagtttg tacaaaaaag ctgaacgaga
     1561 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1621 ataatactgt aaaacacaac atatccagtc atctatggcg gcccctcgag gggccgcatt
     1681 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
     1741 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     1801 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     1861 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     1921 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     1981 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     2041 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     2101 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     2161 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     2221 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     2281 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     2341 gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     2401 acaacagtac tgcgatgagt ggcagggcgg ggcgtaaacg cgtggatccg gcttactaaa
     2461 agccagataa cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga
     2521 tatgtatacc cgaagtatgt caaaaagagg tgtgctatga agcagcgtat tacagtgaca
     2581 gttgacagcg acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg
     2641 taagcacaac catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa
     2701 atcaggaagg gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg
     2761 agaacaggga ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat
     2821 cgtctgtttg tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc
     2881 cccctggcca gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg
     2941 catatcgggg atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc
     3001 gttatcgggg aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt
     3061 aacctgatgt tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt
     3121 cgaccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa
     3181 aatctaattt aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta
     3241 caaagtggtt gataacagcg cttagagctc gaatttcccc gatcgttcaa acatttggca
     3301 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     3361 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     3421 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     3481 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     3541 ggttccggaa ccaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     3601 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     3661 tgagtgagct aactcacatt aggctgaatt aggcgcgcct atttctgaat tcaacacatt
     3721 gcggacgttt ttaatgtact gaattaacgc cgaattaatt cgggggatct ggattttagt
     3781 actggatttt ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa
     3841 tacatactaa gggtttctta tatgctcaac acatgagcga aaccctatag gaaccctaaa
     3901 ttcccttatc tgggaactac tcacacatta ttatggagaa actcgaagtt agtatatagt
     3961 ccttccatca ccctctgtga taatctcctt aaaacttgct cccccaggaa tcataggcag
     4021 aacgtgctcc tggtggggaa ctatcacatc cagtaaataa ggaccgggag aatctagcat
     4081 agtttgaatt gctgctctta gttcatcctt cttcaccaca cgcgcatttg gcaccttgaa
     4141 accgtctgca actgtacaga agtcggggta gatctgctcc tcgtgatctg gatcacctaa
     4201 gtaagtatgt gctctgttgg ccttgtagaa tctatcctcc aattgtacga ccattcctag
     4261 atgttggttg tttaagatca tgatcttcac gggtaacttc tccacaaaaa ttgtagccaa
     4321 ctcctgtacg ttcatcacaa agctaccatc gccatcgatg tcgatcacag gaatatctgg
     4381 ttgagttgcg gcagccccca aagcagaagg aagtccgaat cccatggctc caagacctcc
     4441 agatgtcaac caacgccgcg gttcgttgta cttgaaccac tgggctgccc acatttgatg
     4501 ttggccaaca ccagtagata tgatggcatt tccaccagtg agttcgtaca gcgtctgtat
     4561 ggcagattga ggagcaatgg catcctcgaa tacgggaaac ttcattggcc atttctgctt
     4621 ttgttcttct agttctgcca cccagttgga gaagtcgaaa gtcctctttt gactcttcaa
     4681 gagggcattg atttcactta gcgcaggttt cacatcagca cacatggaca catggggctg
     4741 tttgttcttt ccaatttcag cagggtcaat atcgatatga acgatgctgg acctgctagc
     4801 aaaagtctcc agtttaccag tcactcgatc gtcgaaacgc actccaaatg ccaacaacag
     4861 atctgcttgg tccacggcgt agttggcgta aacagttcca tgcattccaa gcattttcaa
     4921 acttaatgga tcagactcgg gatacgctcc caaacccatt aaagtcgatg tgataggaat
     4981 tccggtaagc tcgacaaatt ccctcaactc ttcgctggcg tgattgcaac ctcctccaga
     5041 atacactaca ggcttcttag aactagaaat gagtcttata atctgctcga ttaacgttgg
     5101 ttccggtggc cgaggcagtc gttgtaagta gcccttcagt tgcattggct gagaccaatc
     5161 tggaatagcc atttgctgtt gcacgtcctt gggaatatct accaagactg ggcccggcct
     5221 acctgaagct gccaaaaaga aagcctccct gattacgcga gggatgtcat caatgttcat
     5281 aacaaggtaa ttgtgcttgg tgatcgatct cgtaacctcc acaattggag tttcttgaaa
     5341 agcatcggta ccaatcatcc tactggatac ttgacctgta atggccacta gtggcacact
     5401 gtccaagagg gcatcagcga gcccggtaac aagattggtc gctccaggac cggatgtggc
     5461 tatacaaacc cctactcgtc cagacgattt agcataacct tcagcagcga aaatctcccc
     5521 ctgctcgtgg cgacagagaa tgttgcggat gctgctggaa cgtgtaagag cctggtgaat
     5581 ctccatagac gctccgccgg gataagcgaa caccgttcta atcccttctc gctcaagagc
     5641 ctccaccaaa atgtcggctc ctttgcgggg ctcgtcatcc ttgtagcgag actgaaagtc
     5701 ggacggggtt ggtgcaacga gtgacttccc gctgctaagc cgtggtccac tcacatcgac
     5761 tgcagactgg atcttctcgc taacagcttg ggctttcact tccagtgaac gctcagtctt
     5821 ccgagaggtg ttgtatgcat gtcccctcag agcggctccc gagaagaaac cactcctcac
     5881 ctttagctct ttagctccct tgcaacaaga ccttgccgat ggctgcacct tctccaggct
     5941 cgctatgctc aaaggcacag ctgcgcatcc actaattgac gccatgctcg agagagatag
     6001 atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg aacttcctta
     6061 tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
     6121 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
     6181 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
     6241 agcctttcct ttatcgcaat tatggcattt gtaggtgcca ccttcctttt ctactgtcct
     6301 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
     6361 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
     6421 gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc cacttgcttt gaagacgtgg
     6481 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
     6541 tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg
     6601 tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc
     6661 cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg
     6721 agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttggcaa
     6781 gctgctctag ccaatacgca aaccgaattc agaaataaat tcagcctaat tcggcgttaa
     6841 ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta
     6901 caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa
     6961 aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg
     7021 taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc
     7081 cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag
     7141 cgttgctgcc tgtgatcaat tcgggcacga acccagtgga cataagcctg ttcggttcgt
     7201 aagctgtaat gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc
     7261 agcggtggta acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta
     7321 cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg
     7381 ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc
     7441 atgggggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc
     7501 gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc
     7561 ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa
     7621 acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc
     7681 gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt
     7741 tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt
     7801 atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa
     7861 catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag
     7921 gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct
     7981 ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc
     8041 aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat
     8101 cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc
     8161 tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta
     8221 gtcggcaaat aatgtctagc tagaaattcg ttcaagccga cgccgcttcg cggcgcggct
     8281 taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag
     8341 tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca
     8401 tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8461 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8521 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8581 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     8641 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8701 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8761 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8821 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8881 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8941 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     9001 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     9061 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     9121 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9181 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9241 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     9301 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     9361 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     9421 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     9481 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct
     9541 tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg
     9601 cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg
     9661 gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg
     9721 cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa
     9781 gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac
     9841 cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc
     9901 tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct
     9961 gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc
    10021 tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc
    10081 aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc
    10141 ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct
    10201 gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc
    10261 aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg
    10321 tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg
    10381 atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg
    10441 gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc
    10501 aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg
    10561 tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat
    10621 tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg
    10681 gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca
    10741 gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg
    10801 cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc
    10861 ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga
    10921 tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg
    10981 gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt
    11041 accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc
    11101 cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
    11161 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
    11221 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
    11281 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
    11341 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
    11401 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
    11461 tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt
    11521 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
    11581 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
    11641 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
    11701 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
    11761 taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa
    11821 tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc
    11881 cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc
    11941 ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac
    12001 tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg
    12061 ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa
    12121 ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac
    12181 cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt
    12241 ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag
    12301 gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc
    12361 gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg
    12421 cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg
    12481 gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg
    12541 ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct
    12601 ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg
    12661 agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg
    12721 gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc
    12781 atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg
    12841 ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg
    12901 tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc
    12961 tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc
    13021 gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag
    13081 cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa
    13141 gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg
    13201 gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact
    13261 gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt actttgatcc
    13321 cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa acct
//
