LOCUS       sequence-134042-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, Phe79>Trp/Cys87>Ser mutant (TdPurE2-F79W,C87S).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Kappock
  TITLE     Serine mutants of Treponema denticola PurE2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK662
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             585..602
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(655..673)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      669..716
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(840..859)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgcgtccg ctggtgatta tccttatggg
       61 cagcagttca gatatgggtc acgccgagaa aattgcgtct gaattgaaaa cttttggtat
      121 tgagtatgcc attcgcatcg gctccgcgca taaaacagcg gagcatgtag tgtctatgct
      181 gaaagaatat gaagctttgg accgtcctaa actgtacatc acgatcgctg ggcgtagtaa
      241 cgctttatca ggctttgttg atggctgggt caaaggtgca accatcgcga gcccaccgcc
      301 aagcgatagc ttcgccggcg ccgacattta ttcttcgtta cggatgccga gcgggattag
      361 tccggcactg gttcttgaac cgaaaaatgc ggcactcctg gcggctcgta ttttttcact
      421 gtacgataaa gaaatcgcag attcggtcaa atcctatatg gaatccaatg cgcagaaaat
      481 tatcgaagac gattcgaaac tgaaacgctg aattcagcgt ttcagtttct tgtcgacgga
      541 gctcgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac caccaccacc
      601 actgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      661 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      721 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
      781 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
      841 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
      901 aaatcggggg c
//
