LOCUS       sequence-13405-e       10640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   Moore AT, Rankin KE, von Dassow G, Peris L, Wagenbach M, Ovechkina 
            Y, Andrieux A, Job D, Wordeman L
  TITLE     MCAK associates with the tips of polymerizing microtubules.
  JOURNAL   J Cell Biol. 2005 May 9. 169(3):391-7.
  PUBMED    15883193
REFERENCE   2  (bases 1 to 10640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2005 May 9. 169(3):391-7."
COMMENT     Sequence Label: ch-Tog
FEATURES             Location/Qualifiers
     source          1..10640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        620..638
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(1455..1472)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7141..7160)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7142..7160)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    7434..7555
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7471..7490)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7525..7544
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7562..8017)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7699..7720)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7911..7930
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8044..8148
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8150..8507
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8150..8341
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8173..8193)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8358..8493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8542..9336
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8542..9336
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8596..8615)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9206..9225
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9524..9543)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9568..9615
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9944..10532
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10433..10452
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc
      661 gcgggaattc taaggaaaac ctggaagcac aatgggagat gacagtgagt ggttgaaact
      721 gccagttgat cagaaatgtg aacacaagct gtggaaagca aggttaagtg ggtatgaaga
      781 ggccctgaag atcttccaga aaataaagga tgaaaagagc ccagagtggt ccaaattttt
      841 aggattgatc aaaaaatttg tcactgattc caatgcagtg gttcaattga aaggattaga
      901 agctgcactt gtttatgttg aaaatgccca tgtagcagga aaaaccacag gagaagttgt
      961 gtcaggtgtt gtaagtaagg tgttcaatca acctaaagct aaagccaagg agctgggcat
     1021 agagatctgt cttatgtaca tagagattga gaaaggagag gctgttcaag aagagctcct
     1081 gaaaggcttg gacaataaga atcccaagat catagtggcc tgtatagaga cactgaggaa
     1141 agccttaagt gaatttggtt ccaaaatcat cttgcttaag ccaattatca aagtgttgcc
     1201 aaaactcttt gagtctcgag agaaggctgt tcgagatgaa gccaaactaa ttgctgtgga
     1261 gatttacaga tggattcggg atgctctgag acccccatta caaaatataa actctgttca
     1321 gttgaaagaa ctagaagaag aatgggtcaa actgccaaca agtgctccta gacctactcg
     1381 atttcttcgt tcccaacaag aactagaagc taaattggaa caacaacagt ctgctggtgg
     1441 agatgctgaa ggaggtggtg atgatggtga tgaggtgcca caaatagatg cttatgagct
     1501 tttagaagct gtagaaatcc tttccaaact tcccaaagac ttttatgaca aaattgaggc
     1561 aaaaaaatgg caagagagaa aagaggccct ggagtctgta gaagtactaa taaaaaaccc
     1621 caaactggaa gctggcgatt atgcagattt agtaaaagca ttaaagaagg ttgttggaaa
     1681 ggacaccaat gtcatgttgg tggctttggc agcaaaatgt cttactggcc tggctgttgg
     1741 gctaaggaag aaatttggac aatatgcagg acatgttgtg ccaaccatct tggagaaatt
     1801 caaagagaag aaacctcaag tggtacaagc cctgcaggag gcaattgatg caatcttcct
     1861 tactaccaca ctacagaaca tcagtgagga tgttttagca gtaatggata ataaaaatcc
     1921 aaccatcaag cagcagacat ctctttttat tgcaagaagt ttccgccact gcactgcttc
     1981 taccctgcca aagagcttgc taaagccctt ttgtgctgca ctacttaagc acatcaatga
     2041 ttctgctcct gaagtcagag atgccgcatt tgaagcattg ggtactgctt tgaaggtggt
     2101 tggcgagaaa gcagtaaaac cattcctagc tgatgtggac aaactcaagc ttgataagat
     2161 caaagaatgt tcagaaaagg tagaactgat acatggtaag aaagctggac tagctgctga
     2221 taagaaggaa ttcaaacctc tgcctggaag gactgctgct tcaggggctg caggagataa
     2281 ggacacaaag gacatttctg cacccaaacc aggacctcta aaaaaggcac ctgctgctaa
     2341 ggctggtggg ccaccaaaaa aggggaaacc agctgcacca ggaggcgcag ggaatactgg
     2401 aaccaagaac aagaaaggac tggagactaa agaaatagtg gagcctgagc tctcgataga
     2461 agtatgcgaa gaaaaagctt cagctgttct tccccctacc tgtatacagc ttcttgacag
     2521 cagtaactgg aaagaaaggc tggcttgtat ggaagagttc cagaaggctg ttgagctaat
     2581 ggaccgaact gaaatgccat gccaggcatt agtgaggatg ctagccaaga aacctggatg
     2641 gaaagaaact aattttcagg tgatgcaaat gaagcttcat atagttgctt tgattgccca
     2701 gaagggaaat ttttccaaaa cgtcagctca ggttgtatta gatggccttg tggacaagat
     2761 tggagatgtg aaatgtggga acaatgcaaa agaagctatg acagcaatag ccgaagcctg
     2821 tatgttacca tggactgctg aacaggttgt gtcaatggct ttctcacaaa agaatcccaa
     2881 aaatcagtca gaaactctga attggctatc aaatgccata aaagaatttg gtttttctgg
     2941 gttgaatgtc aaagctttca ttagcaatgt gaagacagct cttgctgcaa caaacccagc
     3001 tgtgaggact gctgccataa ccctgcttgg cgtgatgtat ctgtatgttg gtccctcttt
     3061 gcgaatgttc tttgaggatg agaagcctgc cctcctatcc cagatagatg cagaatttga
     3121 gaagatgcag ggacaaagtc cacctgctcc aaccagagga atttccaagc atagcacaag
     3181 tggtacagat gaaggagaag atggagatga accagatgac gggagcaatg atgtcgttga
     3241 tcttttgccg aggacggaga tcagtgataa aatcacttca gagttggtat ctaagattgg
     3301 tgataagaat tggaagatta ggaaagaagg cctagatgaa gtggcaggta ttattaatga
     3361 cgcaaaattt atccaaccga atataggtga acttccaact gccttgaagg gtcgactcaa
     3421 tgattcaaat aaaatcttgg tacagcaaac gctgaatatc ctgcaacaac tggcagtagc
     3481 catgggccca aatattaagc aacatgtaaa aaatttaggc atccctatca tcacagtcct
     3541 tggagacagc aagaacaatg ttcgagctgc tgccctagcg actgtgaatg cttgggcaga
     3601 acagactggc atgaaggaat ggctggaagg agaagatctt tctgaagagc tcaaaaagga
     3661 aaatcctttc ttgaggcaag agcttctggg ctggctggct gagaaactac ctactcttcg
     3721 ttccacccct acagacctta tcctttgtgt tcctcatctc tactcctgcc tagaagatcg
     3781 aaatggagat gtgcgaaaga aggcccaaga tgccttgcca ttcttcatga tgcatttagg
     3841 atatgaaaaa atggccaagg ctactgggaa actaaagcca acttctaaag atcaggtatt
     3901 ggccatgcta gagaaagcca aagttaacat gccagccaag cctgctccac ccactaaagc
     3961 aacttctaaa ccaatgggag ggtccgctcc agccaaattc cagcctgcat cagcacctgc
     4021 tgaagattgt atttccagca gtacagaacc caaacctgat ccaaaaaagg ccaaagctcc
     4081 aggattatcc tctaaagcaa agagtgcaca agggaagaag atgccaagca aaaccagctt
     4141 aaaggaggat gaagacaaat ccgggcctat ttttattgtt gttccaaatg gaaaagagca
     4201 aaggatgaaa gatgaaaaag gattgaaggt gctaaagtgg aattttacta ccccacggga
     4261 tgaatacatt gagcaactaa agactcaaat gtctagctgt gtggctaaat ggttacaaga
     4321 tgagatgttt cactcagact ttcagcatca taacaaagcc cttgctgtta tggttgatca
     4381 cttggagagt gaaaaagaag gagttattgg ttgcctggat cttatcttaa agtggcttac
     4441 cctgaggttt tttgacacca atacaagcgt cctgatgaaa gcactagaat atttaaaatt
     4501 gctcttcacc ttgctaagtg aagaagaata tcatcttact gagaatgaag catcttcctt
     4561 catcccctat cttgtcgtca aggttggaga accaaaggat gtcattcgta aagatgttcg
     4621 tgccatcctg aaccggatgt gccttgtcta cccagctagc aagatgtttc cctttatcat
     4681 ggaaggaacc aaatccaaaa actctaagca gagagcagag tgcctggaag agctgggatg
     4741 tctggttgag tcctatggca tgaatgtttg ccaaccaacc ccaggaaaag ccttaaagga
     4801 aatagctgtt cacataggag accgtgacaa tgctgtacgc aatgctgcac tcaacaccat
     4861 tgtaacggtg tacaatgtac atggggatca ggtgttcaaa ctgattggaa atctttctga
     4921 aaaggatatg agcatgctcg aggagaggat taagcggtca gcaaagagac cctctgctgc
     4981 accaataaaa caggtggaag agaaacctca gcgtgcacag aacataagct ccaatgccaa
     5041 catgttacgc aagggaccag ctgaggacat gtcttccaaa ctcaaccaag cccgaagcat
     5101 gagtgggcat cctgaggcag cccagatggt ccgccgagaa ttccagctgg atctagatga
     5161 gattgagaat gacaatggta cagtccgatg tgaaatgcca gaacttgttc agcacaaact
     5221 ggatgacatt tttgagccag tccttattcc tgaacccaag atccgggctg tttctccaca
     5281 cttcgatgac atgcacagta atacagcatc cacaatcaat ttcattatct cccaagtagc
     5341 cagtggtgac atcaacacaa gtatccaagc tctgacacag ctgtttcaga tagagagcct
     5401 tgcccgggag gcctccactg gagtactaaa agacctaatg catggcctca tcaccttaat
     5461 gctggattct cggattgaag atcttgagga aggacaacag gtcatccgct ctgtgaacct
     5521 cttggtggtg aaggttctgg agaagtcaga ccagaccaac atcctgagtg ccctacttgt
     5581 tttgctccaa gacagcctgc tagcaacagc cagttctccc aaattctcag agcttgttat
     5641 gaagtgtctc tggagaatgg ttcgactgtt gcctgatacc atcaatagca ttaacctaga
     5701 cagaattctt ctggatatcc acattttcat gaaggtcttc cccaaagaga aactgaagca
     5761 atgcaaaagt gaatttccca taaggaccct aaagaccctg ctacacacct tatgcaaatt
     5821 aaaagggccc aagatcctgg accacctaac gatgatcgac aacaaaaacg agtctgagct
     5881 ggaggcccat ctctgccgga tgatgaagca cagtatggac cagactggga gcaagtctga
     5941 taaggaaaca gcaaagggag catctcgaat agatgcaaaa tcatcaaagg ccaaagtgaa
     6001 tgatttctta gctgagattt ttaagaagat tggctctaaa gaaaacacta aagagggact
     6061 agcagagtta tatgaatata agaagaaata ctcagatgct gacattgaac catttctgaa
     6121 aaattcctca cagttcttcc agagctatgt cgaaagaggc cttcgggtga ttgagatgga
     6181 gagggagggc aaaggtcgta tttccacttc aacaggcatc tcccctcaga tggaagtcac
     6241 atgtgtgccc acgcccacaa gcacagtgtc ctccataggt aacacaaatg gggaagaagt
     6301 ggggccatct gtctacttgg aaaggctaaa gatcctccga cagcgatgtg gtctggacaa
     6361 cacaaagcaa gatgaccgac ctcctttgac ctctttgctc tccaaaccag cagttcctac
     6421 tgtcgcctct tccacagaca tgctccacag caaactctct cagctccggg agtcacggga
     6481 gcagcaccag cattcagacc tggattctaa ccagactcac tcttcaggaa ctgtgacctc
     6541 ctcctcctcc acagctaaca tagacgactt gaaaaaaaga ctggagagaa taaagagcag
     6601 tcgcaaatga agctgcccca ctcccccggc accctgcagc tttagtttac taaactagaa
     6661 gtcctcatag tttaaaatgg cctcagcagg cctagtgtat acaaactggt tgtatgtatc
     6721 atgccgtgga gctaggggga ggggtcattg tggcacaagt atttgtacat actctgcttc
     6781 tctctgtcag cgtcctgctg ctctagaaga ctgtccgtgg atgagtttag tgtacagact
     6841 tgtaaacagc tgccccctct ctgctcagtc tagttcccag gtccttttct tttcttttta
     6901 attgctcatt tgtaaaattg tcctaatctt tcctagcttt ttaatagtta atattagaaa
     6961 ctctttaata gttttccttt cagtttgtga gctcttctct gtcgccctga agggtcactg
     7021 tattctgtat gaatccatgg catgatacaa ctaatttaag agtcttttat aaataaagtt
     7081 tgcattaact aaaaaaaaaa aaaaaaaaaa aaaaggtacc aggtaagtgt acccaattcg
     7141 ccctatagtg agtcgtatta caattcactc gatcgccctt cccaacagtt gcgcagcctg
     7201 aatggcgaat ggagatccaa tttttaagtg tataatgtgt taaactactg attctaattg
     7261 tttgtgtatt ttagattcac agtcccaagg ctcatttcag gcccctcagt cctcacagtc
     7321 tgttcatgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     7381 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     7441 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7501 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg
     7561 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     7621 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     7681 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     7741 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     7801 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     7861 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     7921 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     7981 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat
     8041 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8101 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagaatcc tgaggcggaa
     8161 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     8221 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     8281 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     8341 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8401 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8461 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag
     8521 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     8581 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     8641 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     8701 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
     8761 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     8821 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     8881 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     8941 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     9001 gatcaggatg atctggacga agaacatcag gggctcgcgc cagccgaact gttcgccagg
     9061 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     9121 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     9181 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agaacttggc
     9241 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     9301 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     9361 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     9421 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     9481 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     9541 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     9601 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     9661 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     9721 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     9781 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     9841 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     9901 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9961 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    10021 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    10081 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    10141 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    10201 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    10261 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    10321 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    10381 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    10441 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    10501 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    10561 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    10621 tggataaccg tattaccgcc
//
