LOCUS       sequence-134053-         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, Lys41>Met mutant (TdPurE2-K41M).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Kappock
  TITLE     Serine mutants of Treponema denticola PurE2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK687
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             520..537
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(590..608)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      604..651
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(775..794)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gttcagatat gggtcacgcc gagaaaattg cgtctgaatt gaaaactttt ggtattgagt
       61 atgccattcg catcggctcc gcgcacatga cagctgagca tgtagtgtct atgctgaaag
      121 aatatgaagc tttggaccgt cctaaactgt acatcacgat cgctgggcgt agtaacgctt
      181 tatcaggctt tgttgatggc ttcgtcaaag gtgcaaccat tgcctgccca ccgccaagcg
      241 atagcttcgc cggcgccgac atttattctt cgttacggat gccgagcggg attagtccgg
      301 cactggttct tgaaccgaaa aatgcggcac tcctggcggc tcgtattttt tcactgtacg
      361 ataaagaaat cgcagattcg gtcaaatcct atatggaatc caatgcgcag aaaattatcg
      421 aagacgattc gaaactgaaa cgctgaattc agcgtttcag tttcttgtcg acggagctcg
      481 aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga
      541 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      601 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      661 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
      721 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      781 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      841 gggggctccc tttagggttc cgatttagtg ctttacggca
//
