LOCUS       sequence-134055-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, Lys41>Arg mutant (TdPurE2-K41R).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Kappock
  TITLE     Serine mutants of Treponema denticola PurE2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK688
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             589..606
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(659..677)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      673..720
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(844..863)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatgcg tccgctggtg attatcctta
       61 tgggcagcag ttcagatatg ggtcacgccg agaaaattgc gtctgaattg aaaacttttg
      121 gtattgagta tgccattcgc atcggctccg cgcatagaac agctgagcat gtagtgtcta
      181 tgctgaaaga atatgaagct ttggaccgtc ctaaactgta catcacgatc gctgggcgta
      241 gtaacgcttt atcaggcttt gttgatggct tcgtcaaagg tgcaaccatt gcctgcccac
      301 cgccaagcga tagcttcgcc ggcgccgaca tttattcttc gttacggatg ccgagcggga
      361 ttagtccggc actggttctt gaaccgaaaa atgcggcact cctggcggct cgtatttttt
      421 cactgtacga taaagaaatc gcagattcgg tcaaatccta tatggaatcc aatgcgcaga
      481 aaattatcga agacgattcg aaactgaaac gctgaattca gcgtttcagt ttcttgtcga
      541 cggagctcga attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac
      601 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      661 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      721 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      781 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      841 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      901 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      961 aaaaacttga ttagggtgat gg
//
