LOCUS       sequence-134056-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Treponema denticola 5-aminoimidazole ribonucleotide 
            carboxylase, Lys41>Arg mutant (TdPurE2-K41R).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Kappock
  TITLE     Serine mutants of Treponema denticola PurE2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK688
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             612..634
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(665..684)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(666..684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttcctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc
       61 ggccgcaagc ttgtcgacgg agctcgaatt cgagctccgt cgacaagaaa ctgaaacgct
      121 gaattcagcg tttcagtttc gaatcgtctt cgataatttt ctgcgcattg gattccatat
      181 aggatttgac cgaatctgcg atttctttat cgtacagtga aaaaatacga gccgccagga
      241 gtgccgcatt tttcggttca agaaccagtg ccggactaat cccgctcggc atccgtaacg
      301 aagaataaat gtcggcgccg gcgaagctat cgcttggcgg tgggcaggca atggttgcac
      361 ctttgacgaa gccatcaaca aagcctgata aagcgttact acgcccagcg atcgtgatgt
      421 acagtttagg acggtccaaa gcttcatatt ctttcagcat agacactaca tgctcagctg
      481 ttctatgcgc ggagccgatg cgaatggcat actcaatacc aaaagttttc aattcagacg
      541 caattttctc ggcgtgaccc atatctgaac tgctgcccat aaggataatc accagcggac
      601 gcatatgtat atctccttct taaagttaaa caaaattatt tctagaggga aaccgttgtg
      661 gtctccctat agtgagtcgt attaatttcg cgggatcgag atctcgggca gcgttgggtc
      721 ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg
      781 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg
      841 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta
      901 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg
      961 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc
     1021 ctgagtgatt tttctctggt cccgccgc
//
