LOCUS       sequence-134062-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Streptococcus pyogenes Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE
  TITLE     Enhancing homology-directed genome editing by catalytically active 
            and inactive CRISPR-Cas9 using asymmetric donor DNA.
  JOURNAL   Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
  PUBMED    26789497
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481."
COMMENT     Sequence Label: pCR1002
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             41..63
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             83..112
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ttaagcggcc gccctgcagg actcgagttc tagaaataat tttgtttaac tttaagaagg
       61 agatatacat atgaaatctt ctcaccatca ccatcaccat caccatcacc atggttcttc
      121 tatgaaaatc gaagaaggta aactggtaat ctggattaac ggcgataaag gctataacgg
      181 tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca ccgttgagca
      241 tccggataaa ctggaagaga aattcccaca ggttgcggca actggcgatg gccctgacat
      301 tatcttctgg gcacacgacc gctttggtgg ctacgctcaa tctggcctgt tggctgaaat
      361 caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg ccgtacgtta
      421 caacggcaag ctgattgctt acccgatcgc tgttgaagcg ttatcgctga tttataacaa
      481 agatctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg ataaagaact
      541 gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact tcacctggcc
      601 gctgattgct gctgacgggg gttatgcgtt caagtatgaa aacggcaagt acgacattaa
      661 agacgtgggc gtggataacg ctggcgcgaa agcgggtctg accttcctgg ttgacctgat
      721 taaaaacaaa cacatgaatg cagacaccga ttactccatc gcagaagctg cctttaataa
      781 aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg tccaacatcg acaccagcaa
      841 agtgaattat
//
