LOCUS       sequence-134064-         167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Streptococcus pyogenes Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 167)
  AUTHORS   Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE
  TITLE     Enhancing homology-directed genome editing by catalytically active 
            and inactive CRISPR-Cas9 using asymmetric donor DNA.
  JOURNAL   Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
  PUBMED    26789497
REFERENCE   2  (bases 1 to 167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481."
COMMENT     Sequence Label: pCR1002
FEATURES             Location/Qualifiers
     source          1..167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(112..132)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cagaaccttg aactttttag acggaacctt atattcatca gtgatcaccg cccatccgac
       61 gctatttgtg ccgatatcta agcctattga gtatttctta tccattgcat tggattggaa
      121 gtacaggttt tcctcgatcc cgaggttgtt gttattgtta ttgttgt
//
