LOCUS       sequence-134068-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of eiF4A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  TITLE     Dreyfuss Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.1+ eiF4A1 myc HIS
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(34..63)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 atggtgatgg tgatgatgac cggtatgcat attcagatcc tcttctgaga tgagtttttg
       61 ttcgaagggc cctctagact cgagcggccg ccactgtgct ggatatctgc agaattcgat
      121 gaggtcacga acattgaggg gcatttcctc aatggaggtg ttgtagaagg tctcaatgtc
      181 tcgaagagtc ctcttgtctt cttctgtcac catgttaata gccacacctt tacggccaaa
      241 ccgtccacct cgaccgattc tgtggatata gttttccctg ttggtgggaa ggtcatagtt
      301 gatgactaaa gaaacctgct gcacatcaat gcctctggcc agcaggtcag tggtaatcaa
      361 aactctgcta gagccagaac gaaactccct cataatcacg tctcgttcct tttggtccat
      421 atctccatgc atggcggata cagtgaaatc tcgagcatgc atcttctcgg tgagccagtc
      481 caccttcctc cgggtgttga tgaagatgac tgcctgggtg atggtcaggg tttcatacaa
      541 gtcacatagt gtgtccagct tccactcctc tcgttccacg ttgatgtaga actggcggat
      601 accctccagg gtcaactctt ccttcttgac aagaatccga atggggtccc tcatgaactt
      661 cttggtcacc tcaagcacat cagaaggcat tgtggctgac agcaaaacta cctgggtgtt
      721 gctgttgagc ttttgga
//
