LOCUS       sequence-134070-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Streptococcus pyogenes dCas9 (D10A, H840A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE
  TITLE     Enhancing homology-directed genome editing by catalytically active 
            and inactive CRISPR-Cas9 using asymmetric donor DNA.
  JOURNAL   Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
  PUBMED    26789497
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481."
COMMENT     Sequence Label: pCR1003
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttcagctttc gttaaattat caaacttacg ttgagtgatt aacttggcgt ttagaagttg
       61 tctccaatag tttttcatct ttttgactac ttcttcactt ggaacgttat ccgatttacc
      121 acgattttta tcagaacgcg ttaagacctt attgtctatt gaatcgtctt taaggaaact
      181 ttgtggaaca atggcatcga catcataatc acttaaacga ttaatatcta attcttggtc
      241 cacatacatg tctcttccat tttggagata atagagatag agcttttcat tttgcaattg
      301 agtattttca acaggatgct ctttaagaat ctgacttcct aattctttga taccttcttc
      361 gattcgtttc atacgctctc gcgaattttt ctggcccttt tgagttgtct gattttcacg
      421 tgccatttca ataacgatat tttctggctt atgccgcccc attactttga ccaattcatc
      481 aacaactttt acagtctgta aaataccttt tttaatagca gggctaccag ctaaatttgc
      541 aatatgttca tgtaaactat cgccttgtcc agacacttgt gctttttgaa tgtcttcttt
      601 aaatgtcaaa ctatcatcat ggatcagctg cataaaattg cgattggcaa aaccatctga
      661 tttcaaaaaa tctaatattg ttttgccaga ttgcttatcc ctaataccat taatcaattt
      721 tcgagacaaa cgtccccaac cagtataacg
//
