LOCUS       sequence-134074-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two 
            C-terminal SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE
  TITLE     Enhancing homology-directed genome editing by catalytically active 
            and inactive CRISPR-Cas9 using asymmetric donor DNA.
  JOURNAL   Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
  PUBMED    26789497
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481."
COMMENT     Sequence Label: pCR1056
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gctttcgtta aattatcaaa cttacgttga gtgattaact tggcgtttag aagttgtctc
       61 caatagtttt tcatcttttt gactacttct tcacttggaa cgttatccga tttaccacga
      121 tttttatcag aacgcgttaa gaccttattg tctattgaat cgtctttaag gaaactttgt
      181 ggaacaatgg catcgacatc ataatcactt aaacgattaa tatctaattc ttggtccaca
      241 tacatgtctc ttccattttg gagataatag agatagagct tttcattttg caattgagta
      301 ttttcaacag gatgctcttt aagaatctga cttcctaatt ctttgatacc ttcttcgatt
      361 cgtttcatac gctctcgcga atttttctgg cccttttgag ttgtctgatt ttcacgtgcc
      421 atttcaataa cgatattttc tggcttatgc cgccccatta ctttgaccaa ttcatcaaca
      481 acttttacag tctgtaaaat acctttttta atagcagggc taccagctaa atttgcaata
      541 tgttcatgta aactatcgcc ttgtccagac acttgtgctt tttgaatgtc ttctttaaat
      601 gtcaaactat catcatggat cagctgcata aaattgcgat tggcaaaacc atctgatttc
      661 aaaaaatcta atattgtttt gccagattgc ttatccctaa taccattaat caattttcga
      721 gacaaacgtc cccaaccagt ataacggcga cgtttaa
//
