LOCUS       sequence-134075-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express Streptococcus pyogenes dCas9 (D10A, H840A) carrying two 
            C-terminal SV40 NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Richardson CD, Ray GJ, DeWitt MA, Curie GL, Corn JE
  TITLE     Enhancing homology-directed genome editing by catalytically active 
            and inactive CRISPR-Cas9 using asymmetric donor DNA.
  JOURNAL   Nat Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481.
  PUBMED    26789497
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jan 20. doi: 10.1038/nbt.3481."
COMMENT     Sequence Label: pCR1056
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      137..146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(150..170)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(228..251)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 agattttttt tgatactgtg gcggtctgta tttcccagaa ccttgaactt tttagacgga
       61 accttatatt catcagtgat caccgcccat ccgacgctat ttgtgccgat agctaagcct
      121 attgagtatt tcttatccat ggtggcattg gattggaagt acaggttttc ctcgatcccg
      181 aggttgttgt tattgttatt gttgttgttg ttcgagctcg aattagtctg cgcgtctttc
      241 agggcttcat cgacagtctg acgaccgctg gcggcgttga tcaccgcagt acgcacggca
      301 taccagaaag cggacatctg cgggatgttc ggcatgattt cacctttctg ggcgttttcc
      361 atagtggcgg caatacgtgg atctttcgcc aactcttcct cgtaagactt cagcgctacg
      421 gcacccagcg gtttgtcttt attaaccgct tccagacctt catcagtcag cagatagttt
      481 tcgaggaact cttttgccag ctctttgttc ggactggcgg cgttaatacc tgcgctcagc
      541 acgccaacga acggtttgga tggttgaccc ttgaaggtcg gcagtaccgt tacaccataa
      601 ttcactttgc tggtgtcgat gttggaccat gcccacgggc cgttgatggt catcgctgtt
      661 tcgcctttat taaaggcagc ttctgcgatg gagtaatcgg tgtctgcatt catgtgtttg
      721 tttttaatca ggtcaaccag gaaggtcaga cccgctttcg cgccagcgtt atccacgccc
      781 acgtcttt
//
