LOCUS       sequence-134077-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human codon-optimized dCas9-DNMT3A fusion with 
            T2A-EGFP for targeted DNA methylation in mammalian cells; cloning 
            backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-EGFP
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcctcgacgt caccgcatgt tagcagactt cctctgccct ctccactgcc gaattcgggc
       61 cggccggaca cacacgcaaa atactccttc agcggagcga agaggtggcg gatgactggc
      121 acgctccatg accggcccag cagtctctgc ctcgccaagc ggctcatgtt ggagacgtca
      181 gtatagtgga ctgggaaacc aaataccctt tccatttcag tgcaccataa gatgtcctct
      241 ttctcattca tgaacacagg aaaatgctgg tctttgccct gctttatgga gtttgacctc
      301 gtagtaatgg tcctcacttt gctgaacttg gctatcctgc catgctccag acactcctgc
      361 agctccagct tatcattcac agtggatgcc aacggcctgt tcataccggg aaggttaccc
      421 cagaagtagc gggccctgtg tgcagctgac acttctttgg catcaatcat cacagggttg
      481 gactcgagaa atcgcgagat gtccctcttg tcactaacgc ccatggccac cacattctca
      541 aagagccaga agaaggggcg atcatctccc tccttgggcc gcgcatcatg caggaggcgg
      601 tagaactcaa agaagagccg gccagtgccc tcgtagaggc ccttgcgagc agggttgacg
      661 atggagaggt cattgcaggg actgccccca atcaccagat cgaatgggcc ccactcctgg
      721 atatgcttct gtgtgacgct gcggacgtcc
//
