LOCUS       sequence-134080-         384 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human codon-optimized dCas9-DNMT3A fusion with 
            T2A-EGFP for targeted DNA methylation in mammalian cells; cloning 
            backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-EGFP
FEATURES             Location/Qualifiers
     source          1..384
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcagcagctg ccgccagtag ttcttcatct tcttcacgac ctcttcggag ggcacgttgt
       61 cgctcttgcc ccggttcttg tcgcttctgg tcagcacctt gttgtcgatg gagtcgtcct
      121 tcagaaagct ctgaggcacg atagcgtcca catcgtagtc ggacagccgg ttgatgtcca
      181 gttcctggtc cacgtacata tcccgcccat tctgcaggta gtacaggtac agcttctcgt
      241 tctgcagctg ggtgttttcc acggggtgtt ctttcaggat ctggctgccc agctctttga
      301 tgccctcttc gatccgcttc attctctcgc ggctgttctt ctgtcccttc tgggtggtct
      361 ggttctctct ggccatttcg atca
//
