LOCUS       sequence-134081-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human codon-optimized dCas9-DNMT3A fusion with 
            T2A-PuroR for targeted DNA methylation in mammalian cells; cloning 
            backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..129
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        206..281
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        378..663
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa
       61 gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat
      121 gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg
      181 aaacaccggg tcttcgagaa gacctgtttt agagctagaa atagcaagtt aaaataaggc
      241 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttgtt ttagagctag
      301 aaatagcaag ttaaaataag gctagtccgt ttttagcgcg tgcgccaatt ctgcagacaa
      361 atggctctag aggtacccgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      421 aacgaccccc gcccattgac gtcaatagta acgccaatag ggactttcca ttgacgtcaa
      481 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      541 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcattg tgcccagtac
      601 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      661 atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc c
//
