LOCUS       sequence-134082-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, 
            Francois JA, Goodman RA, Kappock TJ
  TITLE     Biochemical and structural studies of N5-carboxyaminoimidazole 
            ribonucleotide mutase from the acidophilic bacterium Acetobacter 
            aceti.
  JOURNAL   Biochemistry. 2006 Jul 11;45(27):8193-208.
  PUBMED    16819818
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2006-07-11"; volume: "45(27)"; pages: 
            "8193-208"
COMMENT     Sequence Label: pJK315
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             629..646
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(699..717)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      713..760
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(884..903)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatga tgagcgaaac cgcccccctg
       61 ccctctgctt ccagtgcttt agaagataaa gcagcctctg cccccgttgt gggcattatc
      121 atgggcagcc agtcagactg ggaaaccatg cgccatgcag acgcattgct gacagagctg
      181 gaaattccgc atgaaaccct cattgtttca gcacatcgta cgccagaccg gttggcggat
      241 tatgcgcgta cggcagccga acgcggcctg aatgtgatta ttgctggggc cggtggagct
      301 gcaggtctgc cgggtatgtg tgcggcatgg acacgcctgc ccgtgctggg tgttccagta
      361 gaatctcgcg ctcttaaagg catggacagc ctgctttcca ttgtgcagat gcccggcggc
      421 gtgcctgtgg gcactttggc tattggggca tccggcgcca aaaatgccgc cttgctggcc
      481 gcctccattc ttgcactgta taacccggcc cttgccgcac gccttgaaac atggcgcgca
      541 ttgcagacgg cctctgtccc caactctccc attaccgaag ataaatgaga attcgagctc
      601 cgtcgacaag cttgcggccg cactcgagca ccaccaccac caccactgag atccggctgc
      661 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      721 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      781 cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
      841 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
      901 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
      961 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     1021 ggttcacgta
//
