LOCUS       sequence-134085-         307 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of human codon-optimized dCas9-DNMT3A fusion with 
            T2A-PuroR for targeted DNA methylation in mammalian cells; cloning 
            backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR
FEATURES             Location/Qualifiers
     source          1..307
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgccgccagt agttcttcat cttcttcacg acctcttcgg agggcacgtt gtcgctcttg
       61 ccccggttct tgtcgcttct ggtcagcacc ttgttgtcga tggagtcgtc cttcagaaag
      121 ctctgaggca cgatagcgtc cacatcgtag tcggacagcc ggttgatgtc cagttcctgg
      181 tccacgtaca tatcccgccc attctgcagg tagtacaggt acagcttctc gttctgcagc
      241 tgggtgtttt ccacggggtg ttctttcagg atctggctgc ccagctcttt gatgccctct
      301 tcgatcc
//
