LOCUS       sequence-134088-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mutant human codon-optimized dCas9-DNMT3A fusion 
            (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning 
            backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR (ANV)
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctctctcgg ccttggtcag attgtcgaac tttctctggg taatcagctt ggcgttcagc
       61 agctgccgcc agtagttctt catcttcttc acgacctctt cggagggcac gttgtcgctc
      121 ttgccccggt tcttgtcgct tctggtcagc accttgttgt cgatggagtc gtccttcaga
      181 aagctctgag gcacgatagc gtccacatcg tagtcggaca gccggttgat gtccagttcc
      241 tggtccacgt acatatcccg cccattctgc aggtagtaca ggtacagctt ctcgttctgc
      301 agctgggtgt tttccacggg gtgttctttc aggatctggc tgcccagctc tttgatgccc
      361 tcttcgatcc gcttcattct ctcgcggctg ttcttctgtc ccttctgggt ggtctggttc
      421 tctctggcca tttcgatcac gatgttctcg ggcttgtgcc ggcccatcac tttcacgagc
      481 tcgtccacca ccttcactgt ctgcaggatg cccttcttaa tggcggggct gccggccaga
      541 ttggcaatgt gctcgtgcag gctatcgccc tggccggaca cctgggcttt ctggatgtcc
      601 tctttaaagg tcaggctgtc gtcgtggatc agctgcatga agtttctgtt ggcgaagccg
      661 tcggacttca ggaaatccag gattgtcttg ccggactgct tgtcccggat gccgttgatc
      721 agcttccggc tcagcctgcc ccagccggtg tatctccgcc gcttcagctg cttcatcact
      781 ttgtcgtcga acaggtgggc
//
