LOCUS       sequence-134090-         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mutant human codon-optimized dCas9-DNMT3A fusion 
            (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning 
            backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR (ANV)
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..44
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             51..71
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actacaagga tcatgatatt gattacaaag acgatgacga taagatggcc ccaaagaaga
       61 agcggaaggt cggtatccac ggagtcccag cagccgacaa gaagtacagc atcggcctgg
      121 ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca
      181 agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag
      241 ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa
      301 gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga
      361 tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg
      421 ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg
      481 agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg
      541 acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga
      601 tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc cagctggtgc
      661 agacctaca
//
