LOCUS       sequence-134093-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mutant human codon-optimized dCas9-DNMT3A fusion 
            (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning 
            backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-EGFP (ANV)
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     103..122
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        199..274
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        371..656
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
       61 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
      121 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
      181 gggtcttcga gaagacctgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
      241 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt gttttagagc tagaaatagc
      301 aagttaaaat aaggctagtc cgtttttagc gcgtgcgcca attctgcaga caaatggctc
      361 tagaggtacc cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      421 cccgcccatt gacgtcaata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      481 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      541 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttgtgcccag tacatgacct
      601 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg
      661 aggtgagccc cacgttctgc ttcactctcc cca
//
