LOCUS       sequence-134099-        8889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expresses the androgen receptor, which is required 
            for the activation of the promoter in pLAREG (Addgene plasmid 
            #73045) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Mazumder M, McMillen DR
  TITLE     Design and characterization of a dual-mode promoter with activation 
            and repression capability for tuning gene expression in yeast.
  JOURNAL   Nucleic Acids Res. 2014 Aug;42(14):9514-22. doi: 10.1093/nar/gku651.
            Epub 2014 Jul 23.
  PUBMED    25056312
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku651"; journalName: "Nucleic Acids Res"; date: 
            "2014-08"; volume: "42"; issue: "14"; pages: "9514-22"
COMMENT     Sequence Label: pRR-AR
FEATURES             Location/Qualifiers
     source          1..8889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     70..89
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(308..412)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    449..952
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     994..1012
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1050..1072)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1172..1191
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1353..1996
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     1890..1913
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     promoter        4772..4919
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             4920..5594
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(5691..6146)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5828..5849)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6040..6059
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6272..6294
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6286..6303
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6287..6303
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6310..6329
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6310..6328
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(6481..6668)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     6589..6613
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7175..7191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7175..7191)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7188..7210)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7199..7215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7223..7229,7230..7247,7248..7253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7268..7289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7406..7423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7577..8165)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7657..7676)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8336..8889,1..238,239..307))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
       61 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
      121 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
      181 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      241 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      301 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      361 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      421 cccgaaaagt gccacctggg tccttttcat cacgtgctat aaaaataatt ataatttaaa
      481 ttttttaata taaatatata aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa
      541 tagtttttgt tttccgaaga tgtaaaagac tctaggggga tcgccaacaa atactacctt
      601 ttatcttgct cttcctgctc tcaggtatta atgccgaatt gtttcatctt gtctgtgtag
      661 aagaccacac acgaaaatcc tgtgatttta cattttactt atcgttaatc gaatgtatat
      721 ctatttaatc tgcttttctt gtctaataaa tatatatgta aagtacgctt tttgttgaaa
      781 ttttttaaac ctttgtttat ttttttttct tcattccgta actcttctac cttctttatt
      841 tactttctaa aatccaaata caaaacataa aaataaataa acacagagta aattcccaaa
      901 ttattccatc attaaaagat acgaggcgcg tgtaagttac aggcaagcga tccgtcctaa
      961 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     1021 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     1081 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     1141 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     1201 caccataaac gacattacta tatatataat ataggaagca tttaatagac agcatcgtaa
     1261 tatatgtgta ctttgcagtt atgacgccag atggcagtag tggaagatat tctttattga
     1321 aaaatagctt gtcaccttac gagctcagtt tatcattatc aatactcgcc atttcaaaga
     1381 atacgtaaat aattaatagt agtgattttc ctaactttat ttagtcaaaa aattagcctt
     1441 ttaattctgc tgtaacccgt acatgcccaa aatagggggc gggttacaca gaatatataa
     1501 catcgtaggt gtctgggtga acagtttatt cctggcatcc actaaatata atggagcccg
     1561 ctttttaagc tggcatccag aaaaaaaaag aatcccagca ccaaaatatt gttttcttca
     1621 ccaaccatca gttcataggt ccattctctt agcgcaacta cagagaacag gggcacaaac
     1681 aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc tgcctggagt aaatgatgac
     1741 acaaggcaat tgacccacgc atgtatctat ctcattttct tacaccttct attaccttct
     1801 gctctctctg atttggaaaa agctgaaaaa aaaggttgaa accagttccc tgaaattatt
     1861 cccctacttg actaataagt atataaagac ggtaggtatt gattgtaatt ctgtaaatct
     1921 atttcttaaa cttcttaaat tctactttta tagttagtct tttttttagt tttaaaacac
     1981 cagaacttag tttcgacgaa gctcaaggat ggaagtgcag ttagggctgg gaagggtcta
     2041 ccctcggccg ccgtccaaga cctaccgagg agctttccag aatctgttcc agagcgtgcg
     2101 cgaagtgatc cagaacccgg gccccaggca cccagaggcc gcgagcgcag cacctcccgg
     2161 cgccagtttg ctgctgctgc agcagcagca gcagcagcag cagcagcagc agcagcagca
     2221 gcagcagcag cagcagcagc agcagcaaga gactagcccc aggcagcagc agcagcagca
     2281 gggtgaggat ggttctcccc aagcccatcg tagaggcccc acaggctacc tggtcctgga
     2341 tgaggaacag caaccttcac agccgcagtc ggccctggag tgccaccccg agagaggttg
     2401 cgtcccagag cctggagccg ccgtggccgc cagcaagggg ctgccgcagc agctgccagc
     2461 acctccggac gaggatgact cagctgcccc atccacgttg tccctgctgg gccccacttt
     2521 ccccggctta agcagctgct ccgctgacct taaagacatc ctgagcgagg ccagcaccat
     2581 gcaactcctt cagcaacagc agcaggaagc agtatccgaa ggcagcagca gcgggagagc
     2641 gagggaggcc tcgggggctc ccacttcctc caaggacaat tacttagggg gcacttcgac
     2701 catttctgac aacgccaagg agttgtgtaa ggcagtgtcg gtgtccatgg gcctgggtgt
     2761 ggaggcgttg gagcatctga gtccagggga acagcttcgg ggggattgca tgtacgcccc
     2821 acttttggga gttccacccg ctgtgcgtcc cactccttgt gccccattgg ccgaatgcaa
     2881 aggttctctg ctagacgaca gcgcaggcaa gagcactgaa gatactgctg agtattcccc
     2941 tttcaaggga ggttacacca aagggctaga aggcgagagc ctaggctgct ctggcagcgc
     3001 tgcagcaggg agctccggga cacttgaact gccgtctacc ctgtctctct acaagtccgg
     3061 agcactggac gaggcagctg cgtaccagag tcgcgactac tacaactttc cactggctct
     3121 ggccggaccg ccgccccctc cgccgcctcc ccatccccac gctcgcatca agctggagaa
     3181 cccgctggac tacggcagcg cctgggcggc tgcggcggcg cagtgccgct atggggacct
     3241 ggcgagcctg catggcgcgg gtgcagcggg acccggttct gggtcaccct cagccgccgc
     3301 ttcctcatcc tggcacactc tcttcacagc cgaagaaggc cagttgtatg gaccgtgtgg
     3361 tggtggtggg ggtggtggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg
     3421 cggcggcgag gcgggagctg tagcccccta cggctacact cggccccctc aggggctggc
     3481 gggccaggaa agcgacttca ccgcacctga tgtgtggtac cctggcggca tggtgagcag
     3541 agtgccctat cccagtccca cttgtgtcaa aagcgaaatg ggcccctgga tggatagcta
     3601 ctccggacct tacggggaca tgcgtttgga gactgccagg gaccatgttt tgcccattga
     3661 ctattacttt ccaccccaga agacctgcct gatctgtgga gatgaagctt ctgggtgtca
     3721 ctatggagct ctcacatgtg gaagctgcaa ggtcttcttc aaaagagccg ctgaagggaa
     3781 acagaagtac ctgtgcgcca gcagaaatga ttgcactatt gataaattcc gaaggaaaaa
     3841 ttgtccatct tgtcgtcttc ggaaatgtta tgaagcaggg atgactctgg gagcccggaa
     3901 gctgaagaaa cttggtaatc tgaaactaca ggaggaagga gaggcttcca gcaccaccag
     3961 ccccactgag gagacaaccc agaagctgac agtgtcacac attgaaggct atgaatgtca
     4021 gcccatcttt ctgaatgtcc tggaagccat tgagccaggt gtagtgtgtg ctggacacga
     4081 caacaaccag cccgactcct ttgcagcctt gctctctagc ctcaatgaac tgggagagag
     4141 acagcttgta cacgtggtca agtgggccaa ggccttgcct ggcttccgca acttacacgt
     4201 ggacgaccag atggctgtca ttcagtactc ctggatgggg ctcatggtgt ttgccatggg
     4261 ctggcgatcc ttcaccaatg tcaactccag gatgctctac ttcgcccctg atctggtttt
     4321 caatgagtac cgcatgcaca agtcccggat gtacagccag tgtgtccgaa tgaggcacct
     4381 ctctcaagag tttggatggc tccaaatcac cccccaggaa ttcctgtgca tgaaagcact
     4441 gctactcttc agcattattc cagtggatgg gctgaaaaat caaaaattct ttgatgaact
     4501 tcgaatgaac tacatcaagg aactcgatcg tatcattgca tgcaaaagaa aaaatcccac
     4561 atcctgctca agacgcttct accagctcac caagctcctg gactccgtgc agcctattgc
     4621 gagagagctg catcagttca cttttgacct gctaatcaag tcacacatgg tgagcgtgga
     4681 ctttccggaa atgatggcag agatcatctc tgtgcaagtg cccaagatcc tttctgggaa
     4741 agtcaagccc atctatttcc acacccagtg agtacaatct tgatccggag cttttctttt
     4801 tttgccgatt aagaattaat tcggtcgaaa aaagaaaagg agagggccaa gagggagggc
     4861 attggtgact attgagcacg tgagtatacg tgattaagca cacaaaggca gcttggagta
     4921 tgtctgttat taatttcaca ggtagttctg gtccattggt gaaagtttgc ggcttgcaga
     4981 gcacagaggc cgcagaatgt gctctagatt ccgatgctga cttgctgggt attatatgtg
     5041 tgcccaatag aaagagaaca attgacccgg ttattgcaag gaaaatttca agtcttgtaa
     5101 aagcatataa aaatagttca ggcactccga aatacttggt tggcgtgttt cgtaatcaac
     5161 ctaaggagga tgttttggct ctggtcaatg attacggcat tgatatcgtc caactgcatg
     5221 gagatgagtc gtggcaagaa taccaagagt tcctcggttt gccagttatt aaaagactcg
     5281 tatttccaaa agactgcaac atactactca gtgcagcttc acagaaacct cattcgttta
     5341 ttcccttgtt tgattcagaa gcaggtggga caggtgaact tttggattgg aactcgattt
     5401 ctgactgggt tggaaggcaa gagagccccg aaagcttaca ttttatgtta gctggtggac
     5461 tgacgccaga aaatgttggt gatgcgctta gattaaatgg cgttattggt gttgatgtaa
     5521 gcggaggtgt ggagacaaat ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa
     5581 atgctaagaa ataggttatt actgagtagt atttatttaa gtattgtttg tgcacttgcc
     5641 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa
     5701 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     5761 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     5821 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     5881 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     5941 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     6001 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     6061 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     6121 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa ctgttgggaa
     6181 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     6241 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     6301 agtgaattgt aatacgactc actatagggc gaattgggat ccgtgtggaa gaacgattac
     6361 aacaggtatt gtcctctgag gacataaaat acacaccgag attcatcaac tcattgctgg
     6421 agttagcata tctacaattg ggtgaaatgg ggagcgattt gcaggcattt gctcggcatg
     6481 ccggtagagg tgtggtcaat aagagcgacc tcatgctata cctgagaaag caacctgacc
     6541 tacaggaaag agttactcaa gaacaagaat tttcgtttta aaacctaaga gtcactttaa
     6601 aatttgtata cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag
     6661 aaattcgctt atttagaagt gtcaacaacg tatctaccaa cgatttgacc cttttccatc
     6721 ttttcgtaaa tttctggcaa ggtagacaag ccgacaacct tgattggaga cttgaccaaa
     6781 cctctggcga agaagtccaa agctccaccg cggtggcggc cgttacttac ttagagctcg
     6841 tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataccgg gcaggccatg
     6901 tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg
     6961 gatgttcggt ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt
     7021 cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc
     7081 cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact
     7141 cggcctcgac tctagcgaat taattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
     7201 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7261 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7321 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7381 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7441 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7501 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7561 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7621 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7681 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7741 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7801 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7861 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7921 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7981 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     8041 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8101 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8161 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8221 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8281 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8341 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8401 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8461 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8521 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8581 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8641 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8701 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8761 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8821 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8881 ctggtgagt
//
