LOCUS       sequence-134100-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mutant human codon-optimized dCas9-DNMT3A fusion 
            (inactive catalytic domain of DNMT3A) with T2A-EGFP; cloning 
            backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-EGFP (ANV)
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggccttggtc agattgtcga actttctctg ggtaatcagc ttggcgttca gcagctgccg
       61 ccagtagttc ttcatcttct tcacgacctc ttcggagggc acgttgtcgc tcttgccccg
      121 gttcttgtcg cttctggtca gcaccttgtt gtcgatggag tcgtccttca gaaagctctg
      181 aggcacgata gcgtccacat cgtagtcgga cagccggttg atgtccagtt cctggtccac
      241 gtacatatcc cgcccattct gcaggtagta caggtacagc ttctcgttct gcagctgggt
      301 gttttccacg gggtgttctt tcaggatctg gctgcccagc tctttgatgc cctcttcgat
      361 ccgcttcatt ctctcgcggc tgttcttctg tcccttctgg gtggtctggt tctctctggc
      421 catttcgatc acgatgttct cgggcttgtg ccggcccatc actttcacga gctcgtccac
      481 caccttcact gtctgcagga tgcccttctt aatggcgggg ctgccggcca gattggcaat
      541 gtgctcgtgc aggctatcgc cctggccgga cacctgggct ttctggatgt cctctttaaa
      601 ggtcaggctg tcgtcgtgga tcagctgcat gaagtttctg ttggcgaagc cgtcggactt
      661 caggaaatcc aggattgtct tgccggactg cttgtcccgg atgccgttga tcagcttccg
      721 gctcagcctg ccccagccgg tgtatctccg ccgcttcagc tgcttcatca ctttgtcgtc
      781 gaacaggtgg gcataggttt tcagccgttc ctcgatcatc tctctgtcct caaacagtgt
      841 cagggtcagc acgatatctt
//
