LOCUS       sequence-134111-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of dCas9-DNMT3A fusion with T2A-PuroR and specific sgRNA 
            for targeted DNA methylation of BACH2 promoter in human cells; for 
            use as a control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR_BACH2-sgRNA8
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aggccgcctc tctcggcctt ggtcagattg tcgaactttc tctgggtaat cagcttggcg
       61 ttcagcagct gccgccagta gttcttcatc ttcttcacga cctcttcgga gggcacgttg
      121 tcgctcttgc cccggttctt gtcgcttctg gtcagcacct tgttgtcgat ggagtcgtcc
      181 ttcagaaagc tctgaggcac gatagcgtcc acatcgtagt cggacagccg gttgatgtcc
      241 agttcctggt ccacgtacat atcccgccca ttctgcaggt agtacaggta cagcttctcg
      301 ttctgcagct gggtgttttc cacggggtgt tctttcagga tctggctgcc cagctctttg
      361 atgccctctt cgatccgctt cattctctcg cggctgttct tctgtccctt ctgggtggtc
      421 tggttctctc tggccatttc gatcacgatg ttctcgggct tgtgccggcc catcactttc
      481 acgagctcgt ccaccacctt cactgtctgc aggatgccct tcttaatggc ggggctgccg
      541 gccagattgg caatgtgctc gtgcaggcta tcgccctggc cggacacctg ggctttctgg
      601 atgtcctctt taaaggtcag gctgtcgtcg tggatcagct gcatgaagtt tctgttggcg
      661 aagccgtcgg acttcaggaa atccaggatt gtcttgccgg actgcttgtc ccggatgccg
      721 ttgatcagct t
//
