LOCUS       sequence-134113-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain 
            of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 
            promoter; for use as a control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     115..134
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        214..289
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        386..671
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
       61 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
      121 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
      181 ggacgaaaca ccgaatgtag cgattgagag tgtgttttag agctagaaat agcaagttaa
      241 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttgtttt
      301 agagctagaa atagcaagtt aaaataaggc tagtccgttt ttagcgcgtg cgccaattct
      361 gcagacaaat ggctctagag gtacccgtta cataacttac ggtaaatggc ccgcctggct
      421 gaccgcccaa cgacccccgc ccattgacgt caatagtaac gccaataggg actttccatt
      481 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      541 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattgtg
      601 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      661 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tccccc
//
