LOCUS       sequence-134115-         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain 
            of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 
            promoter; for use as a control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gcctctctcg gccttggtca gattgtcgaa ctttctctgg gtaatcagct tggcgttcag
       61 cagctgccgc cagtagttct tcatcttctt cacgacctct tcggagggca cgttgtcgct
      121 cttgccccgg ttcttgtcgc ttctggtcag caccttgttg tcgatggagt cgtccttcag
      181 aaagctctga ggcacgatag cgtccacatc gtagtcggac agccggttga tgtccagttc
      241 ctggtccacg tacatatccc gcccattctg caggtagtac aggtacagct tctcgttctg
      301 cagctgggtg ttttccacgg ggtgttcttt caggatctgg ctgcccagct ctttgatgcc
      361 ctcttcgatc cgcttcattc tctcgcggct gttcttctgt cccttctggg tggtctggtt
      421 ctctctggcc atttcgatca cgatgttctc gggcttgtgc cggcccatca ctttcacgag
      481 ctcgtccacc accttcactg tctgcaggat gcccttctta atggcggggc tgccggccag
      541 attggcaatg tgctcgtgca ggctatcgcc ctggccggac acctgggctt tctggatgtc
      601 ctctttaaag gtcaggctgt c
//
