LOCUS       sequence-134127-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A human codon-optimized SpCas9 fused to first 110 amino acids of 
            human GEMININ and chimeric guide RNA expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Gutschner T, Haemmerle M, Genovese G, Draetta GF, Chin L
  TITLE     Post-translational Regulation of Cas9 during G1 Enhances 
            Homology-Directed Repair.
  JOURNAL   Cell Rep. 2016 Feb 3. pii: S2211-1247(16)00040-1. doi: 
            10.1016/j.celrep.2016.01.019.
  PUBMED    26854237
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2016 Feb 3. pii: S2211-1247(16)00040-1. doi: 
            10.1016/j.celrep.2016.01.019."
COMMENT     Sequence Label: pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(100..175)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(194..442)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(252..271)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(422..442)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(584..603)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgggtacct ctagagccat ttgtctgcag aattggcgca cgcgctaaaa acggactagc
       61 cttattttaa cttgctattt ctagctctaa aacaaaaaag caccgactcg gtgccacttt
      121 ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct aaaacaggtc
      181 ttctcgaaga cccggtgttt cgtcctttcc acaagatata taaagccaag aaatcgaaat
      241 actttcaagt tacggtaagc atatgatagt ccattttaaa acataatttt aaaactgcaa
      301 actacccaag aaattattac tttctacgtc acgtattttg tactaatatc tttgtgttta
      361 cagtcaaatt aattccaatt atctctctaa cagccttgta tcgtatatgc aaatatgaag
      421 gaatcatggg aaataggccc tcacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
      481 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
      541 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      601 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
      661 tccgcctttc tcccttcggg aagcgtggcg ctttctcata
//
